单个肝癌细胞的拉曼光谱分析研究  

Raman Spectrums of Mono-hepatocellular Carcinoma

作　　者：杨文沛[1] 姚辉璐 朱淼[3] 王桂文[2] 彭立新[2] 何敏[3] 黎永青[4] 

机构地区：[1]右江民族医学院物理教研室,广西百色533000 [2]广西科学院生物物理实验室,广西南宁530003 [3]广西医科大学,广西南宁530021 [4]东卡罗莱纳大学物理系,美国北卡莱罗纳27858

出　　处：《激光与红外》2007年第9期824-827,共4页Laser & Infrared

基　　金：广西科学基金项目(桂科基0575027)资助课题

摘　　要：本工作利用激光镊子拉曼光谱系统研究了人正常肝细胞株(LO2)和肝癌细胞株(SMMC-7721)的单细胞拉曼光谱,对于每个细胞在不同部位测三个点。实验结果显示:正常细胞和癌细胞的平均拉曼光谱存在显著差异;癌细胞谱线强度整体变弱;正常细胞的1658cm-1处峰和1450cm-1处峰的强度比值为0.63,癌细胞的为0.99;癌细胞的核酸、蛋白质、脂类等重要生物分子在结构或含量上都发生了不同的改变。用PCA主成分对单个细胞的平均拉曼光谱进行分析,结果发现PCA可以正确区分出正常细胞和癌细胞。这些结果表明,激光镊子拉曼光谱是判断正常肝细胞和肝癌细胞的有效方法。Monocellular Raman spectrums of normal liver cell line（LO2） and carcinoma liver cell line（SMMC-7721） were studied with laser tweezers Raman spectrums system and each cell was investigated in three different points,the result of which showed：significant differences of average Raman spectrums were found between the normal cell and the carcinoma one;And,the spectra line of carcinoma cell became weak at the whole;Additionally,the spectral peak intensity ratio of normal cell at 1658cm^-1 and 1450cm^-1 was 0.63 while that of carcinoma was 0.99;Moreover,the structure and the amount of protein;nucleic acid,lipids and other such molecules had also changed.Average Raman spectrum of monocell was analyzed with the principal component of PCA,the result of which suggested normal cell and carcinoma cell could be differentiated with PCA.All these indicate that judging normal liver cell and carcinoma cell with laser tweezers Ramam spectrums is an efficient method.

关 键 词：光学 显微拉曼光谱 肝癌 激光镊子 主成分分析 

分 类 号：O657.37[理学—分析化学] R735.7[理学—化学]