玉米浸泡液制备苏云金杆菌生物杀虫剂的影响因素研究  

Factors affecting the production of Bacillus thuringiensis biopesticide using corn steepwater as raw material

作　　者：卢娜[1] 周顺桂[1] 常明[1] 倪晋仁[1] 

机构地区：[1]北京大学环境工程系教育部水沙科学重点实验室,北京100871

出　　处：《环境工程学报》2007年第9期126-130,共5页Chinese Journal of Environmental Engineering

基　　金：广东省自然科学基金资助项目(5006694);广东省科技攻关资助项目(2005B10401008)

摘　　要：以玉米淀粉生产过程中的浸泡液为培养基,摇瓶发酵培养苏云金杆菌生物杀虫剂,通过一系列单因子试验,考察了不同培养条件(种子液的种龄、接种量、浸泡液的含固率、初始pH值、摇床转速、发酵温度及发酵时间)对苏云金杆菌在玉米浸泡液中的生长(菌数增长与芽孢形成)以及发酵液的生物毒效的影响。研究表明,在最佳摇瓶培养条件(种子液种龄10 h,接种量2%,浸泡液含固量3%,初始pH值7.0～7.5,摇床转速200 r/min,发酵温度30℃)下发酵48 h,活菌数和活芽孢数分别可达到7.9×108CFU/mL和5.5×108CFU/mL,毒力效价为698.0 IU/μL。本试验可为生物农药的工业化生产提供实用参数。Using corn steepwater as medium, the fermentation process of Bacillus thuringiensis subsp. kurstaki 130-1 was studied in shaking flask cultures. Effects of various conditions on the growth of Bt including viable cell growth, sporulation and entomotoxicity were investigated; and the optimum culture conditions were determined with shaking flask cultures. Results showed that the suitable culture conditions were as follows： seed age 10 h, inoculum quantity 2% , solid percentage of corn steepwater 3% , initial pH 7.0 -7.5, shaking speed 200 r/min, temperature 30 ℃ and culture time 48 h. Under these conditions, viable cells and viable spores counts were 7.9×10^8 CFU/mL and 5.5 ×10^8 CFU/mL respectively, and entomotoxicity was 698.0 IU/μL. This study provided basis for practical production.

关 键 词：玉米浸泡液 苏云金芽孢杆菌 培养条件 生物杀虫剂 

分 类 号：X703[环境科学与工程—环境工程]