激素敏感型单纯性肾病综合征患儿尿液呼吸道病毒表达及其与发病的相关性  

Relationship between Expression of Respiratory Viruses from Urine in Children with Steroid Responsive Simple Nephrotic Syndrome and Its Episode

作　　者：吴瑾[1] 王峥[1] 陶于洪[1] 

机构地区：[1]四川大学华西第二医院儿科,成都610041

出　　处：《实用儿科临床杂志》2007年第17期1296-1298,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(39870747);卫生部科研基金项目资助(98-1-23)

摘　　要：目的探讨呼吸道病毒感染与激素敏感型单纯性肾病综合征(SRSNS)发病的关系。方法采用反转录-聚合酶链反应(RT-PCR)、桥联酶标(APAAP)法,分别对38例SRSNS患儿(急性期28例,缓解期10例)、18例肾炎性肾病、15例继发性肾病、16例毛细支气管炎和15例健康对照儿童尿液呼吸道病毒基因、抗原进行检测。结果SRSNS急性期组尿液呼吸道病毒基因、抗原检出率均明显高于其他各组,差异有统计学意义(Pa<0.05),其中呼吸道合胞病毒(RSV)检出率最高。病毒检出率在是否应用激素间差异无统计学意义。病毒基因与抗原检测结果有较好一致性。结论呼吸道病毒可能是SRSNS重要的触发因素。　Objective To explore the relationship between respiratory viruses infection and the episode of steroid responsive simple nephrotic syndrome（SRSNS）.Methods Thirty-eight children with SRSNS were selected,including the active stage group（n=28）and the remission group（n=10）.The urine from 18 cases of nephritic nephrosis,16 cases of bronchiolitis,15 cases of secondary glomerular diseases and 15 cases of healthy children were served as controls.By using the method of reverse transcriptase-polymerase chain reaction（RT-PCR）and alkaline phosphoesterase-anti alkaline phosphoesterase enzyme-linked assay（APAAP）,viral genes and antigens in urine were assayed,respectively.Results The positive rate of detecting viruses in the active stage group of SRSNS children was higher than that in the remission group and the other control groups（Pa〈0.05）.The respiratory syncytial virus（RSV）was the most common virus in urine from SRSNS at the active stage.The application of prednision showed no effect on the positive rate of viruses gene and antigens.Conclusion Respiratory tract viruses perhaps play an important role of triggering SRSNS.

关 键 词：肾病综合征 呼吸道病毒 硫酸乙酰肝素 尿液 

分 类 号：R726.91[医药卫生—儿科]