重组鸡白细胞介素18(rChIL-18)对MDV感染SPF鸡细胞免疫的影响  

Effects of recombinant chicken interleukin-18 on cellular immunity in SPF chickens infected with MDV in vitro

作　　者：李宏梅[1] 胡敬东[1] 郭慧君[1] 郑玉姝[1] 刘翠艳[1] 田兆菊[1] 赵宏坤[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2007年第5期624-627,共4页Chinese Journal of Veterinary Science

基　　金：山东省科技攻关重大专项资金资助项目(2005GG1109004);山东省优秀中青年科学家科研奖励基金资助项目(2006BS06015);山东农业大学博士科研基金资助项目(23201)

摘　　要：用马立克氏病病毒(MDV)感染5日龄SPF鸡后,取21日龄和35日龄鸡的淋巴细胞,运用3H-TdR掺入法检测T淋巴细胞对ConA和重组鸡白细胞介素18(rChIL-18)的反应,并用MTT法检测NK细胞和CTL对MD肿瘤细胞系CU147的杀伤活性及rChIL-18和IFN-γ对它们的作用。结果显示,SPF鸡感染MDV后淋转水平显著下降,NK细胞、CTL杀伤活性在感染后21日龄时升高,而在35日龄时NK细胞杀伤活性显著下降。rChIL-18对对照组和感染组SPF鸡的淋转水平和杀伤活性均有提高作用,同样IFN-γ也具有提高NK细胞和CTL杀伤活性的作用。To observe the change of T lymphocytes proliferation and the eytotoxieity of natural killer cells and eytotoxie T lymphocytes in SPF chickens infected with MDV and clarify the effect of rChIL-18 on them in infected chickens,^3H-thymidine（^3H- TdR） incorporation assay was used to detect T lymphocytes response to ConA and rChIL-18;The effect of rChIL-18 and IFN on cytotoxicity of NK cells and CTL to target cell, MDCC-MSBCU147 was analyzed by MTT colorimetric assay. The results indicated that T lymphocytes response to ConA in infected chickens decreased significantly and the cytotoxocity of NK cells depressed at 35 days post inoculation （pi） ; but the cytotoxicity of CTL improved at 21 d pi but decreased remarkably at 35 d pi. The T lymphocytes proliferation and the cytotoxicity of NK cells and CTL,in both non-infected and infected chickens, were improved by rChIL-18. It can be concluded that rChIL-18 could improve the non-specific and specific cellular immunity responses in infected chickens and resist the development of tumor cells.

关 键 词：重组鸡白细胞介素-18(rChIL-18) 细胞杀伤活性 马立克氏病病毒 细胞免疫 SPF鸡 

分 类 号：S852.65[农业科学—基础兽医学]