内毒素致兔红细胞膜流动性改变及阳离子A的拮抗效应  

Alteration of endotoxin-induced erythrocyte membrane fluidity and cation A antagonism in rabbits

作　　者：高斌[1] 高洪[2] 严玉霖[2] 

机构地区：[1]云南农业大学食品科技学院,云南昆明650201 [2]云南农业大学动物科技学院,云南昆明650201

出　　处：《中国兽医学报》2007年第5期703-706,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30360079);教育部高等学校骨干教师资助计划(教计司[2000]65号)

摘　　要：采用分子荧光偏振技术测定正常对照组(Ⅰ组)、内毒素组(Ⅱ组)和阳离子A拮抗组(Ⅲ组)中兔红细胞膜流动性的改变。结果显示,Ⅱ组中红细胞膜的荧光偏振度(P)、微粘度(—η)和荧光各向异性(A)在整个试验过程中均高于Ⅰ组(P<0.05或P<0.01),而Ⅲ组中P、—η和A值除处理后0.5 h变化不明显外(P>0.05),在1、3、57、h均低于Ⅱ组(P<0.05或P<0.01)。据此推断,内毒素可使兔红细胞膜的流动性降低,而阳离子A则能减轻内毒素对红细胞膜流动性的破坏,并对内毒素具有一定的拮抗效应。The experiment was aimed at investigating the alteration of erythrocyte membrane fluidity including fluorescence polarization（P）, microviscocity（^-η） and fluorescence anisotropy（A） in the model of endotoxemia induced by endotoxin in rabbits with the fluorescence polarization technique. Twenty-four healthy rabbits were randomly divided into 3 groups： the normal control （group Ⅰ ）, endotoxin treatment group（ group Ⅱ ） and cation A（CA） and endotoxin treatment group（group Ⅲ ）. CA was used to study the antagonistic mechanism of endotoxin on the erythrocyte membrane （ECM）. It was found that the values of P, ^-η and A in group Ⅱ were higher than those of group Ⅰ during the experiment （ P 〈 0.05, P 〈 0.01 ）. Except no significant difference in P, and A at the 0.5 h（ P 〉 0.05）, the significantly decrease in these parameters in group Ⅲ was observed as compared to those in group Ⅱ（ P 〈 0.05, P 〈 0.01） at the 1,3,5 and 7 h. Above results indicated that endotoxin could decrease the erythrocyte membrane fluidity in the endotoxemia which would be one of the important mechanism of the ECM damage, whereas CA possessed a prominent protective effects on injuries of endotoxin-induced ECM and provides a valuable evidence for the use of some antagonistic endotoxin drugs.

关 键 词：兔 内毒素 阳离子A 红细胞膜 膜流动性 

分 类 号：S852.44[农业科学—基础兽医学]