胰岛素、胰高血糖素对体外培养的牛脂肪细胞HSL mRNA和ADPN mRNA丰度的影响  

Effects of insulin and glucagons on abundance of ADPN mRNA and HSL mRNA in bovine adipocyte

作　　者：丛立新[1] 张才[1] 车英玉[2] 刘国文[1] 王哲[1] 

机构地区：[1]吉林大学畜牧兽医学院 [2]吉林农业大学动物科技学院,吉林长春130118

出　　处：《中国兽医学报》2007年第5期741-743,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30571365)

摘　　要：试验在单层培养的新生犊牛脂肪细胞中,分别添加胰岛素(INS)与胰高血糖素(GN),每个激素设置6个梯度3个重复,通过荧光定量PCR技术,观测2种激素对ADPN mRNA与HSL mRNA丰度的影响。结果表明,胰岛素抑制了脂肪细胞ADPN mRNA与HSL mRNA的表达,胰高血糖素促进了ADPN mRNA与HSL mRNA的表达,且ADPNmRNA与HSL mRNA存在相关性。To investigate whether insulin and glucagons affect the expression of ADPN mRNA and HSL mRNA in adipose cell from a newborn calf, six different levels of insulin and glucagons were added to the media. Abundance of ADPN mRNA and HSL mRNA in adipose cells was determined by real-time quantitative RT-PCR. The results showed that the insulin decreased the expression levels of ADPN mRNA and HSL mRNA in adipose cell, and glucagon had an opposite effectiveness. The results also showed that ADPN mRNA and HSL mRNA have in adipose cell a correlativity .

关 键 词：胰岛素 胰高血糖素 牛脂肪细胞 HSLmRNA ADPNmRNA 

分 类 号：S865.5[农业科学—野生动物驯养] Q852.2[农业科学—畜牧兽医]