普罗布考在抗ox-LDL诱导THP-1巨噬细胞凋亡中对CD36、Caveolin-1表达的影响  

Effects of probucol on ox-LDL induced apoptosis and CD36,Caveolin-1 expression in THP-1 macrophages

作　　者：苏波[1] 何慧[2] 罗其富[1] 朱炳阳[1] 廖端芳[1] 

机构地区：[1]南华大学药理学教研室,湖南衡阳421001 [2]南华大学心血管病研究所,湖南衡阳421001

出　　处：《中国药理学通报》2007年第9期1167-1171,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30470719);湖南省教育厅青年基金项目(No99B11);湖南省教育厅资助项目(No04C540)

摘　　要：目的研究普罗布考(Probucol)在抗ox-LDL诱导THP-1巨噬细胞凋亡中对CD36、Caveolin-1表达的影响。方法用流式细胞仪检测细胞凋亡;用RT-PCR、免疫荧光分别检测CD36、Caveolin-1mRNA水平和蛋白表达。结果普罗布考抗ox-LDL诱导THP-1巨噬细胞凋亡;RT-PCR检测发现普罗布考组与ox-LDL组CD36、Caveolin-1mRNA表达无明显差异;免疫荧光检测结果显示普罗布考下调Caveolin-1蛋白表达,但对CD36蛋白的表达没有影响。结论普罗布考在抗ox-LDL诱导THP-1巨噬细胞凋亡中下调Caveolin-1蛋白的表达。Aim To study the effects of probucol on THP-1 macrophage apoptosis and CD36、Caveolin-1 expression induced by ox-LDL.Methods Apoptosis of THP-1 macrophages was determined by flow cytometry analysis.RT-PCR and immunofluorescence were used to detect CD36,Caveolin-1 mRNA level and protein expression respectively.Results Probucol had no effect on mRNA level of CD36,Caveolin-1 in THP-1 macrophages,but it attenuated Caveolin-1 protein expression.Conclusions Probucol can inhibit apoptosis induced by ox-LDL in THP-1 macrophages by down-regulating Caveolin-1 protein expression.

关 键 词：普罗布考 ox-LDL 巨噬细胞 凋亡 CD36 CAVEOLIN-1 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R341[医药卫生—基础医学]