氯唑沙腙对抗HepG2肿瘤细胞的作用研究  

Effects of chlorzoxazone on HepG2 tumor cells

作　　者：范常龙[1] 栾彦[1] 陈燕[1] 张俊[1] 张婉[1] 杨宝峰[1] 董德利[1] 

机构地区：[1]哈尔滨医科大学药学院药理学教研室

出　　处：《中国药理学通报》2007年第9期1198-1202,共5页Chinese Pharmacological Bulletin

基　　金：黑龙江省杰出青年基金;教育部"新世纪优秀人才支持计划"资助

摘　　要：目的研究氯唑沙腙(chlorzoxazone)对HepG2细胞存活和凋亡的影响。方法采用MTT法检测氯唑沙腙对体外培养的HepG2细胞存活率的影响,通过检测LDH释放观察氯唑沙腙对HepG2细胞的致坏死作用,通过TUNEL法评价细胞凋亡率,透射镜评价细胞的超微结构。结果氯唑沙腙在50～500μmol.L-1浓度范围内对HepG2细胞的存活率均有抑制作用,呈明显的剂量依赖效应关系。荧光显微镜和透射电镜下可见典型的肿瘤细胞凋亡改变。氯唑沙腙在100、200、300及500μmol.L-1浓度下作用48h均可诱导HepG2细胞凋亡,具有明显的剂量效应关系。氯唑沙腙100、200、300及500μmol.L-14种浓度分别作用于HepG2细胞24、48及72h,发现以上各种浓度在48h即可诱导细胞凋亡,作用时间越长,凋亡率越高,有明显的时间效应关系。结论氯唑沙腙能够抑制HepG2细胞存活和诱导细胞凋亡。Aim To investigate effects of chlorzoxazone on survival and apoptosis of HepG2 cells.Methods The necrosis of HepG2 cells was evaluated by measurement of LDH release.The effects of chlorzoxazone on survival of HepG2 cells were assayed by MTT dyereduction.The effects of chlorzoxazone on cell apoptosis was analyzed by TUNEL method.The ultrastructure of HepG2 cells was observed by transmission electron microscope.Results Chlorzoxazone at concentrations of 100～500 μmol·L^-1 nhibited survival ratios of HepG2 cells in a dose-dependent manner significantly.Typical apoptotic changes were observed in HepG2 cells under the fluorescence microscope and transmission electron microscope.Apoptosis of HepG2 cells was induced after treatment of chlorzoxazone at concentrations from 100 μmol·L^-1o 500 μmol·L^-1or 48h,which showed obvious concentration-effect relationship.The apoptotic ratios of HepG2 cells were also increased when chlorzoxazone（100,200,300 and 500 μmol·L^-1was treated for 24,48 and 72 h,which showed obvious time-effect relationship.Conclusion Chlorzoxazone inhibited HepG2 cells survival and induced cell apoptosis.

关 键 词：氯唑沙腙 凋亡 HEPG2 Ca^2+激活K^+通道 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]