噻奈普汀对慢性应激大鼠旷场行为和海马α-CaMPKⅡ表达的影响  

Effects of tianeptine on the open-field behavior and the expression of α-CaMPK Ⅱ in the rat hippocampus after chronic stress

作　　者：何强[1] 韩继阳[1] 王旭梅[1] 彭淼[1] 金魁和[1] 

机构地区：[1]中国医科大学附属盛京医院,沈阳110004

出　　处：《中国行为医学科学》2007年第9期776-778,共3页Chinese Journal of Behavioral Medical Science

基　　金：国家自然基金资助项目（30270497）

摘　　要：目的探讨噻奈普汀对慢性应激抑郁症模型大鼠海马钙/钙调素依赖性蛋白激酶Ⅱ（α-CaMPKⅡ）表达的影响。方法采用慢性综合应激制作动物模型,同时给予药物干预,运用western blot法观察大鼠海马α-CaMPKⅡ的表达变化。结果应激后抑郁症组体质量（167.30±11.10）g、水平穿越格数（12.88±6.51）格、竖立次数（5.88±3.23）次、修饰次数（7.13±4.02）次,均显著低于对照组[分别为（273.20±15.3）g、（52.00±12.17）g、（23.70±6.95）g、（11.00±1.94）g;均P〈0.05]。应激后噻奈普汀组大鼠体质量（173.60±20.40）g与抑郁症组无明显差异（P〉0.05）,水平穿越格数（24.00±9.48）格、竖立次数（14.50±3.95）次、修饰次数（10.80±2.04）次均高于抑郁症组（P〈0.05）,但低于对照组（P〈0.05）。抑郁症组大鼠海马α-CaMPKⅡ表达明显减少（P〈0.01）,噻奈普汀组与对照组差异无显著性（P〉0.05）。结论慢性应激时α-CaMPKⅡ表达减少,噻奈普汀通过α-CaMPKⅡ途径发挥神经元保护作用。Objective To observe the effects of tianeptine on the expression of α-CaMPK Ⅱ in the rat hippocampus after chronic stress. Methods Chronic stress rats were treated with tianeptine, then western blot was used to observe the changes of α-CaMPK Ⅱ expression in the hippocampus of rats. Results After the chronic stress, the body weight （ 167.30 ± 11.10） g, the horizontal crossing numbers （ 12.88 ± 6.51 ）, the erection times （5.88 ±3.23） ,the modification times（7.13 ±4.02） of the depression group are less than control group[ （273.2 ± 15.3）g,（52.0 ±12.17）g, （23.70 ±6.95）g,（11.0 ± 1.94） g; P〈0. 05 ]. There is no obvious difference of body weight between group of tianeptine group and depression group （ P 〉0. 05 ）. As for the horizontal crossing numbers （ 24.00 ± 9.48 ）, the erection times （ 14.50 ± 3.95 ）, the modification times （ 10.80 ± 2.04）, they are all more than the depression group（ P〈0. 05） ,but less than the control group（ P 〈 0. 05 ）. Compared to the control group, the α-CaMPK Ⅱ expression of depression group decreased significantly（ P〈0.01 ） ,and the α-CaMPK Ⅱ expression of tianeptine group had no difference（ P 〉0.05 ）. Conclusion The expression of α-CaMPK Ⅱ in hippocampus of chronic stress depression rat decreased. Tianeptine protects neuron by the way of α-CaMPK Ⅱ.

关 键 词：噻奈普汀 慢性应激 α-CaMPKⅡ 海马 

分 类 号：R686[医药卫生—骨科学]