超声心动图引导下闭胸制备犬急性内膜下心肌缺血和梗死模型  

Preparation of Acute Subendocardial Ischemia and Infarction in Closed-chest Dogs Under Echocardiographic Guidance

作　　者：张清阳[1] 邓又斌[1] 刘冰冰[1] 刘娅妮[1] 黎鹏[1] 申屠伟慧[1] 杨好意[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院超声影像科,武汉市430030

出　　处：《中国超声医学杂志》2007年第5期330-333,共4页Chinese Journal of Ultrasound in Medicine

摘　　要：目的探索建立一个新的闭胸式急性内膜下心肌缺血和梗死动物模型。方法不开胸经导管往6只实验犬左冠脉回旋支近端重复注入直径200～300μm聚乙烯醇(PVA)微球。左冠脉主干注射自制声学造影剂进行实时心肌造影,监测微球栓塞后左室壁血流灌注水平。氯化三苯四氮唑染色法确定室壁梗死程度。结果冠脉回旋支平均累积注射(3.43±0.38)×104微球后,心肌造影发现18节段左室壁内膜下心肌灌注异常,其中16节段发生内膜下梗死。结论超声引导下经导管冠脉注射PVA微球可以诱发犬急性内膜下心肌缺血和梗死。Abstract： Objective To develop a new closed-chest animal model of acute subendocardial ischemia/ infarction. Methods In 6 closed-chest anesthetized dogs, polyvinyl alcohol （PVA） microspheres of 200-300μm in diameter were repetitively injected into the proximal left circumflex coronary artery （LCX） via catheter until subendocardial perfusion abnormality was detected by intracoronary real time myocardial contrast echocardiography （MCE） with self-made microbubbles. The extent of myocardial infarct was identified with triphenyl tetrazolium chloride （TTC） finally. Results Subendocardial opacification abnormalities were detected in 18 left ventricular myocardial segments on real-time MCE after several intracoronary injection of microspheres. The total dose of microspheres injected into the LCX of every dog was （3.43±0.38）× 10^4. Finally, subendocardial infarction confirmed by TTC staining occurred in 16 segments of these abnormal perfusion segments. Conclusions Acute subendocardial ischemia/infarction in dogs could be successfully induced by intracoronary injection of PVA microspheres with standard catheterization technique under ultrasound guidance.

关 键 词：动物模型 内膜下心肌缺血 心肌梗死 超声心动图 造影剂 

分 类 号：R-332[医药卫生]