中国对虾糠虾幼体病原菌(非01群霍乱弧菌)的研究  

VIBRIO CHOLERAE (NON-01), A PATHOGEN OF PENAEUS CHINENSIS LARVAE (MYSIS STAGE) DISEASE

作　　者：王立平 张晓华 刘镁 段爱梅 宋晓玲[2] 徐怀恕[3] 

机构地区：[1]山东省海洋药物科学研究所 [2]中国水产科学研究院黄海水产研究所 [3]青岛海洋大学

出　　处：《中国水产科学》1997年第1期45-51,共7页Journal of Fishery Sciences of China

摘　　要：本文对中国对虾糠虾幼体的一种病原菌———非０１群霍乱弧菌作了研究报道。这种病的症状是病虾运动能力差、趋光性弱、镜检发现肠道肿胀。从垂死病虾中分离到５株细菌，经感染健康糠虾幼体得到与病虾相同症状。５株细菌的４７项形态及生理生化特性与霍乱弧菌相同，但不被０１群霍乱弧菌多价血清凝集。血清学分型结果均为不同的ＶＢＯ血清型ＶＢＯ５，１４，２６，４７，５６。用ＤＮＡ霍乱ＣＴ基因探针菌落原位杂交及小鼠肠结扎法测肠毒素，表明菌株有强毒力。分离菌株对小鼠的ＬＤ５０为１５８×１０８个／只。In this paper. Vibrio cholerae (non-01), a pathogen of Penaeus chinesis larvae (mysis stage) disease was studied. The disease was characterized by swelling in intestine. Five strains of bacteria were isolated from the diseased shrimp larvae (in Mysis). The challenge experiments showed that these isolates were the pathogens of the disease. The isolates were tested for 47 unit characters and classified as Vibrio cholerae, but were not agglutinated by 01 antiserum of Vibrio cholerae. Their serotypes were VBO5, 14, 26, 47 and 56 respectively. Detection of cholerae enterotoxin by the method of DNA CT gene hybridization and intestinal ligation of mice showed that the isolated bacteria had strong virulence.

关 键 词：中国对虾 糠虾幼体 弧菌病 

分 类 号：S945.272[农业科学—水产养殖]