紫草多糖对S_(180)荷瘤小鼠红细胞膜流动性与带3蛋白的影响  

Influence of Polysaccharides of Lithospermum Erythrorhizon on Fluidity and Band-3 Protein Level of Red Cells Membrance of S_(180) Mice

作　　者：张秀娟[1] 穆坤[1] 杨姗姗[1] 季宇彬[1] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076

出　　处：《亚太传统医药》2007年第9期30-33,共4页Asia-Pacific Traditional Medicine

摘　　要：目的:观察紫草多糖对S180荷瘤小鼠瘤重、红细胞膜流动性和带3蛋白含量的影响。方法:采用荧光探针DPH标记法测定红细胞膜的荧光偏振度P,计算微黏度(η)与膜脂流动性(LFU);电泳法测定红细胞膜带3蛋白含量。结果:紫草多糖中剂量组抑瘤率达43.05%;且各剂量组对红细胞膜膜脂流动性、带3蛋白均有影响,以中剂量组效果最佳,与对照组相比P<0.01,P<0.05。结论:紫草多糖通过影响荷瘤小鼠红细胞膜流动性和带3蛋白恢复红细胞的正常功能发挥其抗肿瘤作用。Objective： To observe the influences of polysaccharides of Lithospermum erythrorhizon on fluidity and band-3 protein level of red cells membrance of S180 mice. Method： The fluorescence polarization （P） of red cells was determinend by DPH as probe marking and accounted the microviscosity（η） and membrane fluidity（LFU）; the level of band-3 protein was determined by electro- phoretic method. Results： Inhibition rate of tumor weight of the middle dose group was arrived at 43.05 %, and was influenced the erythrocyte membrane fluidity and band-3 protein level （P〈0. 01, P〈0.05）. Conclusion.. The antitumour actions of polysaccharides of Lithospermum erythrorhizon may be influenced the erythrocyte membrane fluidity and band-3 protein function to recover cell normal function and resist tumor action.

关 键 词：紫草多糖 抑瘤率 红细胞膜膜脂流动性 红细胞膜带3蛋白 

分 类 号：R969.1[医药卫生—药理学]