仔猪感染猪等孢球虫后T细胞亚群的动态变化  

Dynamic Observation on the T Lymphocyte Subgroups of Piglets Infected with Isospora suis

作　　者：王芳[1] 宁长申[1] 张龙现[1] 菅复春[1] 朱静静[1] 姚雷[1] 张敏[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002

出　　处：《中国农学通报》2007年第9期24-27,共4页Chinese Agricultural Science Bulletin

基　　金：河南省自然科学基金重点项目"猪球虫病流行病学调查及致病种生物学特性研究"(0311032400)

摘　　要：为了研究仔猪感染猪等孢球虫后T细胞亚群的变化规律,应用纯种猪等孢球虫孢子化卵囊人工感染9头6日龄仔猪,分别检测了感染前1d和感染后1、3、6、9、11d(DPI)外周血中CD3+、CD4+、CD8+T细胞。结果显示,DPI3,CD3+、CD4+T细胞增加,之后下降,但差异均不显著(p>0.05);CD8+T细胞在DPI6增加,DPI 9达峰值(P<0.05)。CD4+/CD8+值在DPI 6下降,DPI 11达到最低点。表明DPI 3感染仔猪的免疫功能有所增强,而DPI 8后仔猪免疫功能受到抑制。In order to reveal the dynamic changes of the T lymphocyte subgroups in piglets, nine six-day-old piglets were inoculated with Isospora suis sporulated occysts. CD3^＋, CD4^＋, CD8^＋ T cells were checked on before and post the day inoculation 1, 3, 6, 9, 11 （DPI）, respectively. Results showed that the amounts of CD3^＋, CD4^＋ T cells increased on DPI 3 and then decreased, but the diversity was not significant （p〉0.05）; however, CD8^＋ T cell raised on DPI 6 and reached peak value on DPI 9 （P〈0.05）. The ratio of CD4^＋/CD8^＋ began to descend on DPI 6, and came to the lowest point on DPI 11. Those indicated that the immunologi- cal functions of the piglets infected with I. suis were reinforced on DPI 3, however, which were suppressed after DPI 8 .

关 键 词：仔猪 猪等孢球虫 CD3+T细胞 CD4+T细胞 CD8+T细胞 

分 类 号：S852.723[农业科学—基础兽医学]