一种快速低廉的PCR探针标记方法  

A Rapid and Twopenny PCR Labeling Method

作　　者：许本波[1] 谢伶俐[1] 柴友荣[2] 李加纳[2] 田志宏[1] 

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]西南大学农学与生物科技学院,重庆400716

出　　处：《中国农学通报》2007年第9期82-84,共3页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金重点项目"甘蓝型油菜优质高效黄籽性状的遗传基础研究"(30330400)

摘　　要：【研究目的】探针标记技术是分子生物学和基因工程研究中常用实验技术,需要发展高效、快速、低成本、安全、简捷的DNA探针标记方法;【方法】利用普通的Taq酶进行PCR探针标记,电泳和Southern杂交检测探针标记结果;【结果】电泳检测发现标记产物在电泳时相对非标记的对照表现明显滞后,表明探针标记成功;电泳条带清晰明亮,表明探针标记效率高;Southern杂交条带清晰,说明该方法标记的探针可用于Southern等分子杂交实验;【结论】利用普通的Taq酶进行PCR探针标记,是一种低成本、高效、快速的DNA探针标记方法。[OBJECTIVE] DNA labeling technology is important for research of molecular and genetic engineering, So it is important to develop a high-efficiency, rapid, lowcost, safe, simple and convenient method for labeling DNA; [METHOD] Based on PCR principle, DNA was labeled using Taq DNA Polymerase and the result was detected by electrophoresis and southern-blot.[RESULTS]The electrophoresis results indicated the product labeled was lag than control DNA, which improved that the label succeed; The bright and clear strip indicated the labeling efficiency was high; Cross strip in southern-blot was clear, which proved the product labeled by the method can be used in molecular hybridization. [CONCLUSIONIPCR labeling using Taq DNA polymerase is a low-cost, high-efficlency and rapid DNA labeling method.

关 键 词：DNA探针标记 TAQ DNA PCR标记 

分 类 号：Q503[生物学—生物化学]