甘草离体培养物中总黄酮提取工艺的优化  

Extraction and Content Determination of Total Flavonoids of Cultures of Glycyrrhiza uralensis Fisch

作　　者：葛淑俊[1] 李广敏[2] 马峙英[1] 王文全[3] 安金翠[1] 孟义江[1] 

机构地区：[1]河北农业大学,保定071001 [2]河北省农林科学院,石家庄050051 [3]北京中医药大学,北京100102

出　　处：《中国农学通报》2007年第9期107-110,共4页Chinese Agricultural Science Bulletin

基　　金：河北省自然科学基金项目"甘草有效成分甘草酸合成的细胞培养技术研究"(C2004000318);河北省教育厅项目"甘草有效成分甘草酸合成的细胞培养技术研究"(2004438)

摘　　要：【研究目的】对甘草离体培养物中总黄酮提取和测定工艺进行优化,以建立在室内筛选高产细胞系和试管苗株系的方法;【方法】采用超声波提取技术,对提取液、浸泡时间、提取温度等进行优化;【结果】将样品以75%甲醇浸泡6h后在32℃下超声提取两次,比色法测定总黄酮含量,加样回收率为95.3%～101.4%,相对标准偏差为1.61%。测得甘草初代愈伤组织中总黄酮含量为0.0012%～0.05%,子叶愈伤组织调控后含量高达0.08%,试管苗中总黄酮含量为1.04%～1.31%,移栽后高达4.5%。【结论】此工艺准确度较高、稳定性好、简便可行,为优良细胞系的筛选和试管苗的早期鉴定奠定了技术基础。[objective] to optimize the extraction and determination method of the total flavonoids thus to screen the cell clones and plantlets with high contents, [method] the ultrasonic technique was used and several treatments were tried and screened, [result] the best condition was marinated 6 hours with 75% carbinol then ultrasonic extracted twice. And then the content was determined with colorimetry from cultures of Glycyrrhiza uralensis Fisch. The result showed the recovery rate was between 95.3% and 101.4% and the RSD was 1.61%. The total flavonoids of the initial calli was between 0.0012% and 0.05%, the im- provement cotyledonary calli was up to 0.08%, the contents of the plantlets were between 1.04% and 1,31%, and after being transplanted was up to 4.5%. [conclusion] The rate of veracity of this method was high and this provided the determination of Glycyrrhiza cultures flavonoids products a feasible method, and those data offered a credible academic gist for authenticate of the experiment resource.

关 键 词：甘草 离体培养物 总黄酮 超声提取 

分 类 号：R284.2[医药卫生—中药学]