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作 者:梁建根[1] 竺利红[1] 吴吉安[1] 桑金隆[1] 姚杭丽[1] 施跃峰[1]
机构地区:[1]浙江省农业科学院,植物保护与微生物研究所,杭州310021
出 处:《中国农学通报》2007年第9期327-330,共4页Chinese Agricultural Science Bulletin
摘 要:为了明确生防菌BH-2的抑菌作用和抑菌因子的相关性质,采用管碟法,对其发酵液中活性代谢产物的抑菌作用及理化性质进行了研究。结果表明,发酵液中活性代谢产物具有较广的抗菌谱,对多种植物病原菌均表现较好的抑制作用。此外,活性代谢产物耐121℃高温,在偏酸性或中性条件下稳定,有较强的抗紫外线照射能力,对蛋白酶K和胰蛋白酶稳定性较好,54℃下15d的稳定性较好。这为该菌株的进一步研究和产品开发奠定了坚实的基础。A study on antifungal action and physicochemical characteristics of bioactive metabolite produced by biocontrol bacterial strain BH-2 fermentation was launched by means of cylinder plate method for the sake of evaluating the antifungal action and relative properties of inhibitive factors of strain BH-2.The results showed that the bioactive metabolite had a broad antifungal spectrum and exhibited antagonistic abilities to several plant pathogens.In addition, it could endure the treatment of a high temperature of 121~C, was stable at acidic, neutral,54℃ for 15d respectively, maintained the antifungal activity after treatment of proteinase K, trypsin and ultraviolet radiation. These provided substantial foundation for a further research and production development of strain BH-2.
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