培养基对尖孢镰刀菌非致病力菌株281产孢量的影响  

Effects of Medium on Sporification of Fusarium oxysporum Schl.

作　　者：蓝江林[1] 朱育菁[1] 肖荣凤[1] 杨淑佳[2] 葛慈斌[1] 刘波[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福州350003 [2]厦门大学生命科学院,厦门361005

出　　处：《中国农学通报》2007年第9期415-418,共4页Chinese Agricultural Science Bulletin

基　　金：福建省自然科学基金"内生生防菌短芽孢杆菌GFP标记及其对黄瓜枯萎病生防机理的研究"(2006J0068);福建省发改委重点项目"生物农药高毒力菌株筛选利用与菌种基因资源库的建立"(闽发改投资[2006]781号)

摘　　要：选择8种培养基,采用液体培养法,研究培养基质对尖孢镰刀菌(Fusarium oxysporum Schl.)281菌株小孢子产生量的影响,结果表明,在PDA培养基上培养144h得到最大小孢子产量1.283×109cfu/ml,与其它培养基产孢量差异显著(P=0.05)。培养至192h时,ATCC培养基的小孢子数量达到1.325×109cfu/ml,与PDA培养基之间无显著差异(P=0.05),与其它6种培养基之间差异显著(P=0.05)。综合比较8种培养基,PDA培养基培养时间短,配制简单,是较为理想的产小孢子培养基。The results of eight mediums effect on sporification of Fusarium oxysporum 281 showed that the number of sporification is 1.283x109cfu/ml in PDA medium after 144h, Significant difference were observed between PDA medium and others （P=0.05）. The number of sporification is 1.325x109cfu/ml in ATCC medium after 192h, there are no significant difference between ATCC and PDA medium（P=0.05）. PDA medium is suitable for sporification.

关 键 词：培养基 尖孢镰刀菌 非致病力菌株 产孢量 

分 类 号：S432.1[农业科学—植物病理学]