转几丁质酶-葡聚糖酶双价基因水稻稻米毒理试验  

Safety Assessment of Transgenic Rice with Chitinase Gene and β-1,3-glucanase Gene

作　　者：姚春馨[1] 许明辉[1] 李进斌[2] 谭学林[3] 田文忠[4] 唐祚舜[4] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,昆明650223 [2]云南省农业科学院植物保护研究所,昆明650205 [3]云南农业大学稻作研究所,昆明650201 [4]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《中国粮油学报》2007年第4期18-23,共6页Journal of the Chinese Cereals and Oils Association

基　　金：国家转基因植物研究与产业化专项JY-03-B-13;国家自然科学基金资助项目(30260059);云南省自然科学基金资助项目(2002C0079M)

摘　　要：通过小鼠急性毒性试验、小鼠致突变试验、大鼠90d毒性试验,对转几丁质酶-葡聚糖酶双价基因水稻稻米的毒理进行了初步的评价。转基因大米粉MTD≥37.5g/kg灌胃给予小鼠,连续观察7d,动物全部存活,表现正常,未见中毒反应。致突变灌胃给予小鼠,未发现对小鼠骨髓细胞微核和精子畸形发生率有不良影响。转基因大米粉分别以15、7.5g/kg/d,90d灌胃大鼠,其行为活动、外观体征、饮食、粪便、体重,结果均无异常,5项血液学检查和13项生化指标检测及尸体解剖观察、脏器系数、脏器病理学检查,结果均属正常,与空白对照无显著差异。转基因大米粉结果提示携带几丁质酶基因、葡聚糖酶基因、NPT-II标记基因等外源DNA的转基因大米无明显的毒性和致畸作用。Safety evaluation of the transgenic rice flour possessing Chitinase gene, β - 1,3 - glucanase gene and foreign DNA sequences including NPTII gene was conducted by urgent toxicity and teratogenicity tests with rats or mice. Results show as follows： All mice administered by enema the solution containing transgenic rice flour at a heavy dose of 37.5g/kg for 7d are alive and no significant toxic effect appears in growth and body weight gain. Ninety-day mice feeding test shows no significant differences in physical sign, growth, body weight gain, 5 hemogram indexes and 13 biochemical indexes of blood, organ relative weights and pathologic anatomy between rats administered the solution containing transgenic rice flour at two doses of 15 and 7.5g/kg and equate volume of 0.9% physiological saline as control by enema. It is revealed that the Chitinase and β - 1,3 - glucanase transgenic products show no toxicity and teratogenicity effect in vivo by the micronucleus and sperm aberration tests at two doses of 15 and 7.5g/kg. These results indicate that the Chitinase and β - 1,3 - glucanase transgenic rice is as safe as existing commercial rice.

关 键 词：转基因水稻 几丁质酶基因 葡聚糖酶基因NPT-Ⅱ基因 安全性评价 毒理试验 

分 类 号：S572[农业科学—烟草工业]