烤烟叶片高光谱参数与多种生理生化指标关系研究  

Relationship Between Hyperspectra Parameters and Physiological and Biochemical Indexes of Flue-cured Tobacco Leaves

作　　者：李向阳[1] 刘国顺[1] 杨永锋[1] 赵春华[1] 喻奇伟[1] 宋世旭[1] 

机构地区：[1]河南农业大学国家烟草栽培生理生化研究基地,郑州450002

出　　处：《中国农业科学》2007年第5期987-994,共8页Scientia Agricultura Sinica

基　　金：国家烟草专卖局重点资助项目(110200401021)

摘　　要：【目的】为了快速、准确的检测烤烟叶片生理生化指标,获取烟田施肥和烤烟生长信息。【方法】通过不同施氮、钾量试验,采用ASD Field spec FR 2500光谱仪,分析不同处理烤烟叶片光谱特性,同时运用相关分析、逐步回归分析等统计方法,研究了不同营养水平下烤烟叶片高光谱遥感特征参数与烟叶生物化学成分(总氮、叶绿素、类胡萝卜素)和生理指标(鲜叶重、干叶重、含水率)之间的关系。【结果】不同氮、钾处理间叶片光谱曲线差异较大、规律明显。叶片多种高光谱参数与10种生理生化指标之间的相关性较强,通过逐步回归方程筛选出了叶片10种生理生化指标的高光谱特征变量,其中与7种生化成分关系最密切的光谱特征变量均是红边面积与蓝边面积比值SDr/SDb,建立的回归方程的的复确定系数和回归系数相伴概率显著性都达到0.05水平,同时通过检测样本的检验,估测效果较好。【结论】因此,可利用这些变量建立的估测模型对烤烟叶片中多种生理生化指标进行快速测定,同时可根据叶片高光谱特性初步监测烟田氮钾施用量和烤烟生长状况。[ Objective] The experiment was conducted to examine the physiological and biological indexes quickly and exactly, and to obtain information of tobacco-field fertilizing and tobacco growing. [Method] We used ASD Field spec FR 2 500 to measure spectra reflectance of flue-cured tobacco and studied the relationship between hyperspectra parameters and biochemical contents （Total nitrogen, chlorophyll, carotenoid） and physiological indexes （Fresh weight, dry weight, moisture content） of flue-cured tobacco leaves by correlation and stepwise regression statistic methods at different nitrogen and potassium levels. [Result] The spectra curves of different treatments had obvious rules and great diversities. There were high correlations between kinds of spectra parameters and ten physiological and biochemical indexes of flue-cured tobacco leaves. Hyperspectra characteristic variables of ten physiological and biochemical indexes were found out through stepwise regression, and SDr/SDb was the closest characteristic variable to seven biochemical contents. Simultaneously, R2 and the regression coefficients of equations reached 0.05 significant level and the equations had good estimating effects through the examination of other samples. [Conclusion] Accordingly, this study suggested that the ten physiological and biochemical indexes could be estimated quickly by the estimating models, at the same time we could inspect nitrogen-potassium fertilization and growth condition of flue-cured tobacco.

关 键 词：烤烟 叶片 高光谱参数 生理生化指标 

分 类 号：S572[农业科学—烟草工业]