抗氰烯菌酯的禾谷镰刀菌nit突变体的诱导及其生物学特性  

Induction and Characterization of Nitrate Nonutilizing Mutants of Fusarium graminearum Resistant to Fungicide JS399-19

作　　者：陈雨[1] 陈长军[1] 王建新[1] 金丽华[1] 周明国[1] 

机构地区：[1]南京农业大学植保学院农业部病虫监测与治理重点实验室,南京210095

出　　处：《中国农业科学》2007年第4期735-740,共6页Scientia Agricultura Sinica

基　　金：高等学校博士学科点专项科研基金(20050307028);国家自然科学基金(30671048);国家自然科学基金(30671384);国家"973"计划(2006CB101900);江苏省科技攻关项目(BG2006328);国家科技支撑计划(2006BAE01A04-08)

摘　　要：【目的】研究抗氰烯菌酯的禾谷镰刀菌nit突变体的生物学特性。【方法】将3个抗氰烯菌酯的禾谷镰刀菌(Fusarium graminearum Schw.)菌株分别在含氯酸盐的MMC培养基上培养,共获得了50个硝酸盐利用缺陷突变体(nit)。比较了抗性菌株的nit突变体与亲本在无性和有性阶段的主要生物学性状。【结果】抗性菌株的nit突变体与亲本在菌落生长速率、培养性状和致病性方面没有显著差异;但某些突变体中,产孢量和产子囊壳能力方面存在一定差异。此外,禾谷镰刀菌对氯酸盐和氰烯菌酯之间没有交互抗药性,且抗性可以稳定遗传。【结论】可以将nit作为遗传标记来研究禾谷镰刀菌对氰烯菌酯的抗药性遗传学。[Objective] Nit mutants of Fusarium graminearum resistant to JS399-19 was characterized. [Method] Fifty nitrate nonutilizing mutants （nit） were obtained by transferring the 3 JS399-19-resistant mutants of Fusarium graminearum onto MMC media. [Result] Results showed that there were no significant differences in mycelial growth rate, cultural characters and pathogenicity between JS399-19-resistant nit mutants and their parental isolates. The conidiophore production and perithecigerous capacity changed more or less in some mutants. Results also indicated that there were no cross-resistance against chlorate and fungicide JS399-19 in Fusarium graminearum, and the resistance to both chlorate and JS399-19 could be transferred by asexual reproduction steadily. [Conclusion] Nit can be used as a genetic marker for genetic studies of Fusarium graminearum resistant to fungicide JS399-19.

关 键 词：禾谷镰刀菌 氰烯菌酯抗药性 生物学性状 遗传标记 

分 类 号：S432.4[农业科学—植物病理学]