大花蕙兰种质资源亲缘关系的RAPD分析  

Analysis on the Genetic Relationships of Hybrid Cymbidium'S Germplasm Resources With RAPD Markers

作　　者：李冬梅[1,2] 叶庆生[3] 朱根发[1] 

机构地区：[1]广东省农业科学院花卉研究所 [2]华南师范大学生命科学学院/广东省植物发育生物工程重点实验室广州510631 [3]华南师范大学生命科学学院/广东省植物发育生物工程重点实验室

出　　处：《中国农业科学》2007年第4期800-806,共7页Scientia Agricultura Sinica

基　　金：广东省科技攻关重大专项(2003A201040);广州市农业局招标项目(GK0302105)

摘　　要：【目的】从分子水平研究大花蕙兰品种资源之间的遗传亲缘关系,为大花蕙兰种质资源的保存和利用及其杂交亲本的选配提供依据。【方法】利用RAPD标记方法对来自日本、韩国、中国和美国的48个大花蕙兰品种和2个国产兰属原生种的种质资源亲缘关系进行了检测。【结果】从100个10碱基随机引物中筛选出20个多态性高的引物,共扩增出258条DNA带,其中253条为多态带(占98.1%),平均每个引物扩增多态性带12.6条。50份种质之间的相似系数变化范围为0.364～0.817,平均相似系数为0.581。基于RAPD的扩增结果建立的UPGMA亲缘关系聚类图,50份种质在相似系数0.592处被划分为5大类群。【结论】供试50份种质间的遗传亲缘关系与其来源地、花色、花枝类型和杂交系谱有一定的相关性。RAPD技术能很好的用于大花蕙兰品种亲缘关系的研究。[Objective] This study researched the genetic relationships of hybrid Cymbidium＇s germplasm resources at molecular levels, and further obtained information for germplasm storage, utilization, and breeding selection parents for breeding. [Method] The random amplified polymorphic DNA marker（RAPD） was assessed to detect the genetic relationships among 48 hybrid Cymbidium cultivars from Japan, Korea, China and USA, and 2 species of native Cymbidium. [Result] 20 primers were screened from 100 random decamer primers, and a total of 258 DNA bands were amplified, 253 of which （98.1%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 12.6. Genetic similarities among the cultivars and species were estimated based on the amount of band sharing ranged from 0.364 to 0.817 with an average of 0.581. According to the data, a dendrogram of genetic relationship which was constructed using UPGMA method showed that all the tested cultivars and species were classified into five cluster groups with the similarity coefficient of 0.592. [Conclusion] It revealed that the genetic relationships among tested accessions were to some extent related with their origin, flower color, branch type and genealogy. It further indicated that RAPD technique is a useful tool for the studying of genetic relationships among hybrid Cymbidium cultivars.

关 键 词：大花蕙兰 亲缘关系 RAPD 聚类分析 

分 类 号：S682.31[农业科学—观赏园艺]