丹酚酸B对脑缺血小鼠脑能荷和三磷酸腺苷酶活性的影响  

Effects of salvianolic acid B on cerebral energy charge and activity of ATPase in mice with cerebral ischemia

作　　者：蒋玉凤[1] 王秋华[1] 刘智勤[2] 王谦[1] 蔡大勇[1] 刘世静[1] 张颖[1] 黄启福[1] 

机构地区：[1]北京中医药大学基础医学院病理教研室,北京100029 [2]河北大学药学院药理教研室,河北保定071002

出　　处：《中国中药杂志》2007年第18期1903-1906,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30472281)

摘　　要：目的:从脑缺血小鼠脑组织高能磷酸含量和三磷酸腺苷(ATP)酶活性探讨丹酚酸B对脑水肿的影响。方法:将NIH小鼠分为假手术组、缺血组、丹酚酸B治疗组和尼莫地平对照组,复制脑缺血模型。丹酚酸B组尾静脉注射丹酚酸B(22.5mg·kg^(-1));尼莫地平组尾静脉注射尼莫地平(0.03mg·kg^(-1));假手术组和缺血组尾静脉注射等量的生理盐水,30min后阻断双侧颈总动脉,观察缺血30min脑组织ATP、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)和磷酸肌酸(PCr)的含量并计算能荷(EC)值;同时测定Na^+-K^+-ATP酶和Ca^(2+)-ATP酶的活性以及脑含水量。结果:与缺血组比较,丹酚酸B组脑组织ATP,ADP,PCr含量和能荷值显著升高(P<0.01);Na^+-K^+-ATP酶和Ca^(2+)-ATP酶活性显著增强(P<0.01);而脑含水量则明显降低(P<0.05)。结论:丹酚酸B减轻脑缺血小鼠脑含水量的作用机制,可能与其提高高能磷酸含量和增强ATP酶活性有关。Objective： To observe the effect of salvianolic acid B （SalB） on high energy phosphate and activity of ATPase of cerebral ischemia in mice, and to study the role of SalB on hydrocephalus further. Method： NIH mice were divided into four groups randomly： Sham-operated group, cerebral ischemia group, SalB-treated group and Nimodipine （Nim）-collated group. In Sal B-treated group, mice were injected with SalB （22. 5 mg · kg^- 1 ） in vena caudalis at 30 min before the experiment. In Nim-collated group, Nim （0. 03 mg · kg^-1 ） was injected into tail vein at the same time, while the mice in Sham-operated group and cerebral ischemia group were injected the same volume normal saline. The acute cerebral ischemia model was established by ligating bilateral common carotid arteries for 30 min in mice, then the mice were killed and the content of adenosine triphosphate （ATP）, adenosine diphosphate （ADP）, adenosine monophosphate （AMP）, phosphocreatine （PCr） were observed, and the cerebral energy charge （EC） was computed. At the same time, activity of Na^＋ -K^＋ -ATPase and Ca^2＋ -ATPase, content of water in brain tissue were measured. Result： Compared with cerebral iscbemia group, EC and content of ATP, ADP, PCr in SalB-treated group heightened evidently （ P 〈 0. 01 ）. Moreover, activity of Na^＋ -K^＋ ATPase and Ca^2＋ ATPase in SalB-treated group had a remarkable increase （P 〈 0. 01 ）. But the content of water in brain tissue decreased markedly （ P 〈 0. 05 ）. Conclusion： The mechanism that SalB can relieve content of water in brain tissue of cerebral iscbemia in mice, may be associated with improving the content of high-energy phosphoric acid compounds and enhancing the activity of ATPase.

关 键 词：脑缺血 能量代谢 丹酚酸B 小鼠 

分 类 号：R285.5[医药卫生—中药学]