黄连解毒汤醇提物预防给药对MDR模型小鼠相关基因表达产物的影响  

Effect on mouse S180 MDR tumour cell expression correlated factorial matter by 70% ethanol with Huanglian Jiedu Tang

作　　者：李贵海[1] 孙付军[1] 陈锋[2] 杨书斌[1] 张军[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东省肿瘤防治研究院,山东济南250117

出　　处：《中国中药杂志》2007年第18期1906-1908,共3页China Journal of Chinese Materia Medica

基　　金：山东省自然科学基金(Y2005C57)

摘　　要：目的:观察黄连解毒汤70%醇提物预防给药对小鼠多药耐药(MDR)基因表达产物P170、肺耐药蛋白(LRP)和拓扑异构酶Ⅱ(TOPOⅡ)的影响,探讨其干预MDR的分子生物学基础,指导临床应用以预防MDR的产生。方法:小鼠随机分为非耐药模型组、耐药模型组、黄连解毒汤70%醇提物100,50mg·kg^(-1)组,模拟临床PFC方案,建立小鼠S180肿瘤细胞多药耐药在体模型,同时给予黄连解毒汤70%醇提物4周,流式细胞仪荧光检测P170,LRP,TOPOII。结果:黄连解毒汤70%醇提物明显降低化疗诱导后耐药细胞P170,LRP的表达率和ROPOII活性。结论:黄连解毒汤70%醇提物可通过对相关生物活性物质的调节,干预和逆转化疗诱发的肿瘤MDR的产生。Objective： To observe the effect on P170, LRP, TOPO Ⅱ of S180 tumour MDR mice for matter by 70% ethanol with Huanglian Jiedu Tang, and then discuss the molecular biology base for chnic. Method： 18-22 gramme KM rats were distributed four groups for normal S180 tumour cell group, matter by 70% ethanol with Huanglian Jiede tang 100 mg · kg^-1 and 50 mg · kg^-1 in random. Every rat was given S180 cell 0.2 mL by celiac, and after 24 hours give Cisplatin for Injective 3 mg · kg^-1, ip, once a week. And give cyclophosphamide and 5-FU 3 mg · kg^-1, ig, once every day. After 15 days, collect lively rats ascites and give it for onefold normal rat. And then repeat before process. At the same time, every group was given corresponding medicine for O. 2 mL · 10 g^-1. The normal group and the model group were given the same cubage water, all together fore weeks. At last observd the P170, LRP, TOPO Ⅱ by flow cytometry. Result： Matter by 70% ethanol with Huanglian Jiedu Tang could obviously reduce the express of P170 and LRP, and the activiation of TOPO Ⅱ. Conclusion： Matter by 70% ethanol with Huanglian Jiedu Tang can intervene the ocurrence of the multi-drug resistance of tumour cells by regulating the biology gene.

关 键 词：黄连解毒汤70%醇提物 肿瘤多药耐药 小鼠 P170 LRP TOPO Ⅱ 

分 类 号：R285.5[医药卫生—中药学]