枳棋子醋酸乙酯提取物对大鼠肝P450同工酶的影响  

Effects of ethyl acetate extract of Semen Hoveniae on liver microsomal cytochrome P450 isoenzyme in rat

作　　者：张洪[1] 宋娟[1] 詹新安[1] 谭晔[1] 

机构地区：[1]武汉大学人民医院药学部,湖北武汉430060

出　　处：《中国中药杂志》2007年第18期1917-1921,共5页China Journal of Chinese Materia Medica

基　　金：湖北省科技攻关项目(2002AA301C61)

摘　　要：目的:观察枳椇子醋酸乙酯提取物对大鼠肝P450同工酶的影响。方法:大鼠分别灌胃给予枳椇子醋酸乙酯提取物相当于生药量0.14,0.17,0.2g·kg^(-1),连续10d,采用紫外分光光度法测定细胞色素P450与NAD- PH-细胞色素C(P-450)还原酶含量及苯胺羟化酶(ANH)、氨基比林N-脱甲基酶(ADM)、红霉素N-脱甲基酶(ERD)的活性。采用RT-PCR技术检测大鼠肝脏CYP1A1,CYP2C11,CYP2E1,CYP3A1基因的水平。结果:枳椇子醋酸乙酯提取物对细胞色素P450及NADPH-细胞色素C(P-450)还原酶含量无显著影响;可抑制苯胺羟化酶(ANH)活性,抑制率可达31.5%;可增加氨基比林N-脱甲基酶(ADM)活性,可达42.4%;而对红霉素N-脱甲基酶(ERD)的活性没有明显变化。在mRNA水平上,CYP2E1基因的表达水平没有变化;CYP1A1,CYP2C11和CYP3A1基因表达水平则明显增加。结论:枳椇子醋酸乙酯提取物对大鼠肝P450同工酶的影响表现出特异性,对不同的P450同工酶作用不同。Objective： To investigate the effects of the ethyl acetate extract of Semen Hoveniae （ESH） on liver microsomal cytochrome P450 isoenzyme in rats. Method： The rats were given orally the ESH in the doses of 0. 14, 0. 17, 0. 2 g · kg （ equivalent to the crude herb） for 10days respectively. Rat liver microsomal cytochrome P450, NADPH-Cyt C reductase, erythromycin N-demethylase （ERD）, Aniline hydroxylase （ANH） , aminopyrine N-demethylase （ADM） activities were quantitated by UV chromatography. The levels of mRNA expression of CYP1A1, CYP2C11, CYP2E1 and CYP3A1 were detected by semi-quantitative reverse transcripatase-polymerase chain reaction （RT-PCR）. Result： The cytochrome P450 content, NADPH-Cyt C reductase activities and erythromycin N-demethylase （ ERD ） activities were not affected. Aniline hydroxylase （ANH） activities in liver were decreased by up to 35. 1% ; aminopyrine N-demethylase （ADM） activitiesin liver were increased by up to 42. 4%. The mRNA expression of CYP1A1, CYP2C11 and CYP3A1 were found to be increased markedly. Conclusion： A specific effect of ESH on liver microsomal cytochrome P450 isoenzyme in rats was observed in this investigation. ESH had various effects on liver microsomal cytochrome P450 isoenzyme.

关 键 词：枳棋子 醋酸乙酯提取物 细胞色素P450 逆转录聚合酶链反应 

分 类 号：R285.5[医药卫生—中药学]