Comparative Analysis of Genomes in Oryza sativa, O. officinalis, and O. meyeriana with C_(0)t-1 DNA and Genomic DNA of Cultivated Rice  

作　　者：LAN Wei-zhen HE Guang-cun WANG Chen-yi WU Shi-jun QIN Rui 

机构地区：[1]College of Life Sciences, Huzhou Teachers College, Huzhou 313000, P.R.China [2]Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, P.R. China [3]Key Laboratory for Plant Developmental Biology, Ministry of Education/College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China

出　　处：《Agricultural Sciences in China》2007年第9期1027-1034,共8页中国农业科学（英文版）

基　　金：This work was supported by the National High Tech R ＆ D Program of China （863 Program, 2004AA227120）; the Scientific Research Foundation for the Returned 0verseas Chinese Scholars, Ministry of Education of China （BZY04003）; China Postdoctoral Science Foundation （20040350574）;the Project of Science and Technology for Youth, Wuhan, China （2004500607135）.

摘　　要：Fluorescence in situ hybridization （FISH） and comparative genomic hybridization （CGH） were applied to somatic chromosomes preparations of Oryza sativa, O. officinalis, and O. meyeriana with labeled probes of Cot-1 DNA and genomic DNA＇from the cultivated rice. The coverage percentage （%） and size （Mb） of Cot-1 DNA in O. sativa, O. officinalis, and O. meyeriana were 47.1 ±0.16, 38.61 ±0.13, 44.38＋_0.13, and 212.33 ± 1.21,269.42 ± 0.89, 532.56± 1.68 Mb, respectively. The coverage percentage and size of genomic DNA from O. sativa in O. officinalis and O. meyeriana were 91.0, 93.6% and 634, 1 123 Mb, respectively, in which 365 and 591 Mb in O. officinalis and O. meyeriana were from O. sativa genomic DNA, but not from repetitive sequences of O. sativa, and the uncoverage genome size in O. officinalis and O. meyeriana were 64 and 78 Mb, respectively. In addition, karyotype analysis was conducted based on the signal bands of Cot-1 DNA in O. sativa, O. officinalis, and O. meyeriana. The results showed that highly and moderately repetitive sequences in Oryza genus were conserved as the functional genes during evolution. The repetitive sequences reduplication may be one of the important causes of the genome enlargement of O. officinalis and O. meyeriana, and O. officinalis genome enlarged more slowly when compared with O. meyeriana. Based on the above results, it is concluded that O. officinalis and O. meyeriana were formed by reduplication, rearrangement, and gene selective loss during the evolution process.Fluorescence in situ hybridization （FISH） and comparative genomic hybridization （CGH） were applied to somatic chromosomes preparations of Oryza sativa, O. officinalis, and O. meyeriana with labeled probes of Cot-1 DNA and genomic DNA＇from the cultivated rice. The coverage percentage （%） and size （Mb） of Cot-1 DNA in O. sativa, O. officinalis, and O. meyeriana were 47.1 ±0.16, 38.61 ±0.13, 44.38＋_0.13, and 212.33 ± 1.21,269.42 ± 0.89, 532.56± 1.68 Mb, respectively. The coverage percentage and size of genomic DNA from O. sativa in O. officinalis and O. meyeriana were 91.0, 93.6% and 634, 1 123 Mb, respectively, in which 365 and 591 Mb in O. officinalis and O. meyeriana were from O. sativa genomic DNA, but not from repetitive sequences of O. sativa, and the uncoverage genome size in O. officinalis and O. meyeriana were 64 and 78 Mb, respectively. In addition, karyotype analysis was conducted based on the signal bands of Cot-1 DNA in O. sativa, O. officinalis, and O. meyeriana. The results showed that highly and moderately repetitive sequences in Oryza genus were conserved as the functional genes during evolution. The repetitive sequences reduplication may be one of the important causes of the genome enlargement of O. officinalis and O. meyeriana, and O. officinalis genome enlarged more slowly when compared with O. meyeriana. Based on the above results, it is concluded that O. officinalis and O. meyeriana were formed by reduplication, rearrangement, and gene selective loss during the evolution process.

关 键 词：Cot-1 DNA CGH KARYOTYPE O. officinalis O. meyeriana 

分 类 号：S511[农业科学—作物学]