血管紧张素Ⅱ对心脏成纤维细胞PTEN基因表达及增殖的影响  

Effect of AngⅡ expression of PTEN gene and proliferation of cardiac fibroblast cells in neonate rats

作　　者：聂凌[1] 王江[1] 宋熔[1] 祝善俊[1] 

机构地区：[1]第三军医大学新桥医院心血管内科,重庆400037

出　　处：《心脏杂志》2007年第4期377-379,共3页Chinese Heart Journal

基　　金：国家自然科学基金项目资助(No.30370584)

摘　　要：目的以AngⅡ刺激乳鼠心脏成纤维细胞(CFC),观察CFC的增殖情况以及第10号染色体同源丢失性磷酸酶张力蛋白基因(PTEN)及转化生长因子-β1(TGF-β1)基因表达。方法以AngⅡ(1×10-7mmol/L)刺激分离培养的CFC,以MTT法检测成纤维细胞的增殖情况,以RT-PCR的方法检测PTEN及TGF-β1基因表达。结果MTT测定的结果:32 h后AngⅡ刺激组的吸光值(A)显著高于该时间点对照组(P<0.05),与正常对照组相比,AngⅡ作用组PTEN基因表达显著下降(P<0.05),TGF-β1基因显著上调(P<0.05)。结论在AngⅡ的刺激下,CFC的TGF-β1表达上调,抑制PTEN基因表达,并引起细胞增殖。AIM To observe the effect of proliferation of cardiac fibroblast cells and expression of PTEN and TGF-β1 gene stimulated by Ang Ⅱ in neonate rats. METHODS Cardiac fibroblast cells （CFCs） were cultured and stimulated by Ang Ⅱ （ 1×10^-7mmol/L）. Cell proliferation was measured by MTT and expressions of PTEN and TGF-β1 gene were measured by RT-PCR. RESULTS MTT absorbance （A） of Ang Ⅱ -stimulated fibroblast cells was significantly higher than that of the control group（ P 〈 0.05 ） after 32 hours. Expression of PTEN increased and expression of TGF-β1 gene decreased compared with those in the control group（P 〈 0.05 ）. CONCLUSION When CFCs in neonate rats are stimulated by Ang Ⅱ, they up-regulate the expression of TGF-β1 gene and down-regulate the expression of PTEN gene, thus promoting the proliferation of the cells.

关 键 词：心脏成纤维细胞 血管紧张素Ⅱ 第10号染色体同源丢失性磷酸酶张力蛋白基因 转化生长因子-Β1 增殖 

分 类 号：R541.6[医药卫生—心血管疾病] R394.33[医药卫生—内科学]