应用基因芯片检测烟草脆裂病毒  

Gene chip detecting for Tobacco rattle virus

作　　者：马新颖[1] 汪琳[1] 任鲁风[2] 邓丛良[1] 高文娜[1] 汪万春[1] 周琦[1] 

机构地区：[1]北京出入境检验检疫局,北京10002 [2]本元正阳基因技术有限公司

出　　处：《植物病理学报》2007年第4期352-355,共4页Acta Phytopathologica Sinica

基　　金：国家"十五"食品安全重大专项资助项目;国家"863"计划资助项目(2003AA2Z2009)

摘　　要：Total RNA in tulips was extracted by Trizol method.Primers were designed according to the sequences of Tobacco rattle virus and 18S rRNA gene of plant.The corresponding sections were amplified by RT-PCR and the PCR products were labeled by Cy3-dCTP.The probes of plant virus,18S rRNA gene and comparisons were designed and immobilized on chips.Labeled PCR products were hybridized with the probes and the signals were scanned by scanner and analyzed by GenePix Pro 4.0 software.Tobacco rattle virus was detected from tulips which were imported from Holand.The accuracy and sensitivity of the plant virus gene chip were proved.Total RNA in tulips was extracted by Trizol method. Primers were designed according to the sequences of Tobacco rattle virus and 18S rRNA gene of plant. The corresponding sections were amplified by RT-PCR and the PCR products were labeled by Cy3-dCTP. The probes of plant virus, 18S rRNA gene and comparisons were designed and immobilized on chips. Labeled PCR products were hybridized with the probes and the signals were scanned by scanner and analyzed by GenePix Pro 4.0 software. Tobacco rattle virus was detected from tulips which were imported from Holand. The accuracy and sensitivity of the plant virus gene chip were proved.

关 键 词：基因芯片 检测 烟草脆裂病毒 有害生物 检疫 

分 类 号：S432.41[农业科学—植物病理学]