芜菁花叶病毒欧亚分离物HC-Pro基因的克隆和序列分析  

Cloning and sequence analysis of HC-Pro genes of Turnip mosaic virus Eurasian isolates

作　　者：施曼玲[1] 

机构地区：[1]杭州师范大学生命与环境科学学院,杭州310036

出　　处：《植物病理学报》2007年第4期383-389,共7页Acta Phytopathologica Sinica

基　　金：浙江省自然科学基金项目(Y305090)

摘　　要：16个芜菁花叶病毒（Turnip mosaic virus，TuMV）欧亚分离物分别来自奥地利、丹麦、德国、匈牙利、尼泊尔和英国6国。利用免疫捕获反转录PCR（Immunocapture RT-PCR，IC-RT-PCR）对16个分离物的HC-Pro（Helper component pro-teinase）基因进行PCR扩增，扩增产物克隆后进行序列测定，HC-PrD基因序列长度均为1374个核苷酸，编码458个氨基酸。16个分离物的HC—PrD基因核苷酸序列同源性为79．5％～99．8％，所编码的氨基酸同源性为94．1％～99．8％。对16个分离物及GenBank上已报道的其它14个TuMV的HC-Pro基因核苷酸的系统进化树分析表明：在16个TuMV欧亚分离物中，除了来自亚洲的分离物N23属Asian-BR组，其余15个来自欧洲的分离物都属于world-B组，其中分离物H1归属world-wide亚组，另外14个分离物则归属NewWorld亚组。Sixteen virus isolates of Turnip mosaic virus （TuMV） were collected from Austria, Denmark, Germany, Hungary, Nepal and United Kingdom, respectively. The HC-Pro （Helper component proteinase） genes of these isolates were amplified by Immunocapture RT-PCR（ IC-RT-PCR）, cloned and determined. HC-Pro genes of the 16 isolates were all composed of 1 374 nucleotides encoding a polypeptide of 458 amino acids sharing 79.5% to 99.8% and 94.1% to 99.8% identities at the nucleotide and the amino acid level, respectively. The phylogenetic trees constructed based on nucleotide sequences of HC-Pro genes of the 16 TuMV isolates together with 14 previously reported TuMV isolates indicate that 15 （ all from Europe） of the 16 Eurasian isolates belong to world-B group, but isolate N23 （only one from Asian） belongs to Asian-BR group. Among the 15 Eurasian isolates in world-B group, isolate H1 belongs to world-wide subgroup, while the other 14 Eurasian isolates belong to New World subgroup.

关 键 词：芜菁花叶病毒 HC-PRO基因 序列分析 

分 类 号：S432.41[农业科学—植物病理学]