洋葱伯克霍尔德氏菌株Lyc2的鉴定及对棉苗的防病促生作用  

Physio-biochemical characterization and molecular identification of Burkholderia cepacia isolate Lyc2 as a PGPR to cotton seedlings

作　　者：于晓庆[1] 郗丽君[1] 刘永光[1] 李光伟[1] 兰玉菲[1] 竺晓平[1] 李向东[1] 

机构地区：[1]山东农业大学植物保护学院植物病理系,泰安271018

出　　处：《植物病理学报》2007年第4期426-432,共7页Acta Phytopathologica Sinica

基　　金：山东省优秀中青年科学家奖励基金(2004BS06002);山东农业大学科学基金资助项目

摘　　要：从烟草根际土壤中分离到一株细菌菌株Lyc2,平皿对峙培养显示该菌可显著抑制多种植物病原真菌菌丝体的生长。温室盆栽试验表明,Lyc2对棉花苗期立枯病的防治效果达到48.8%;水培棉花苗试验表明,Lyc2能显著增加棉苗的鲜重和干重,但对株高的影响不显著。该菌经形态、生理生化试验测定及16S rDNA和ITS序列分析,初步确定为洋葱伯克霍尔德氏菌(Burkholderia cepacia);进一步通过种特异的recA基因序列分析,证明Lyc2菌株属于B. cepacia复合物中基因型IX,B.pyrrocinia。A bacterial strain, Lyc2, was isolated from tobacco rhizosphere and showed remarkable inhibition to the mycelial growth of multiple pathogenic fungi in dual culture. In greenhouse, strain Lyc2 showed 48.8% efficiency to control cotton seedling damping-off. In solution culture experiment, compared with control, Lyc2 significantly increased the fresh weight and dry weight of cotton seedlings. The morphological, physiological and biochemical characteristics of Lyc2 were similar to those of Burkholderia cepacia. Homology analysis of 16S rDNA and ITS sequences showed that Lyc2 was closely related to B. cepacia. Further results of recA gene sequence comparison showed that Lyc2 belonged to B. pyrrocinia, genomovar IX of B. cepacia complex.

关 键 词：植物根际促生菌 洋葱伯克霍尔德氏菌 16S RDNA 16S-23S rDNA转录单元内间隔区序列(ITS) RECA基因 

分 类 号：S482.292[农业科学—农药学] S476[农业科学—植物保护]