小麦蓝矮病植原体转运蛋白secY基因片段序列分析  

SecY gene sequence analysis of phytoplasma associated with wheat blue dwarf

作　　者：张荣[1] 崔晓艳[1] 孙广宇[1] 康振生[1] 

机构地区：[1]西北农林科技大学植物保护学院,杨凌712100

出　　处：《植物病理学报》2007年第4期446-448,共3页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30670013);教育部"长江学者和创新团队发展计划"创新团队项目(200558);陕西省自然科学基金资助项目(2005C23)

摘　　要：Wheat blue dwarf(WBD)is a disease caused by phytoplasma and only reported from China.A fragment about 1.3 kb in protein translocation gene,secY was amplified by PCR from the total DNA of di-seased wheat sample with primer pair secYF/secYR,which was designed based on secY gene sequence of known 16SrI group members.Nucleotide acid sequence analysis of amplified fragment indicated that the length was 1 240 bp.A phylogenetic tree based on secY gene sequences was constructed and showed that wheat blue dwarf phytoplasma was clustered into the Candidatus Phytoplasma asteris,subgroup 16SrI-C.Wheat blue dwarf phytoplasma showed high homology with clover phyllody phytoplasma strains based on sequence comparison and phylogenetic analysis.Wheat blue dwarf （WBD） is a disease caused by phytoplasma and only reported from China. A fragment about 1.3 kb in protein translocation gene, secY was amplified by PCR from the total DNA of diseased wheat sample with primer pair secYF/ secYR, which was designed based on secY gene sequence of known 16SrI group members. Nucleotide acid sequence analysis of amplified fragment indicated that the length was 1 240 bp. A phylogenetic tree based on secY gene sequences was constructed and showed that wheat blue dwarf phytoplasma was clustered into the Candidatus Phytoplasma asteris, subgroup 16SrI-C. Wheat blue dwarf phytoplasma showed high homology with clover phyllody phytoplasma strains based on sequence comparison and phylogenetic analysis.

关 键 词：小麦 蓝矮病 植原体 转运蛋白secY基因片段 序列分析 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]