应力培养对血管内皮细胞形态结构的影响  

THE CULTURE OF ENDOTHELIAL CELLS IN A FLOW ENVIRONMENT

作　　者：江国伟[1] 孙继虎[1] 王克强[1] 于彦铮[1] 郦鸣阳[2] 

机构地区：[1]上海医科大学解剖学教研室,上海200032 [2]上海大学流体控制研究室

出　　处：《解剖学杂志》1997年第3期257-261,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金资助

摘　　要：为在体外形成一个抗流动切应力作用能力强的单层内皮细胞,在15 dynes/cm^2切应力的条件下,培养肉皮细胞24h.结果内皮细胞中央出现应力纤维,G_0/G_1期细胞增加.这一现象提示,在切应力条件下培养肉皮细胞,可增强细胞抗血流冲击的能力.The bovine aortic endothelial cells (ECs) were cultured in a flow environment for 24 h in the parallel plate flow apparatus (shear stress: 15 dynes/cm2, quasi-steady flow). It was found that stress fibers in ECs were induced by shear stress. The formation of endothelial stress fibers in response to shear stress is probably a functionally important mechanism that protects the en-dothelium from hemodynamic detachment. It was also found that the percentage of endothelial cells in G0/G1, phase was significantly increased. This indicates that shear stress can reduce the ECs turnover rate. These results suggest that, in order to achieve a low cell turnover rate and a shear resistant endothelial layer-one comparable to that observed in vivo, it may be necessary to have endothelial cells cultured in a flow environment.

关 键 词：内皮细胞 切应力 应力纤维 血管 细胞形态 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]