12种夹竹桃科植物的RAPD分析  

RAPD analysis of twelve species of Apocynaceae

作　　者：黄久香[1] 张寿洲[2] 庄雪影[1] 李秉滔[1] 

机构地区：[1]华南农业大学林学院,广东广州510642 [2]深圳仙湖植物园,广东深圳518000

出　　处：《福建林业科技》2007年第3期14-18,共5页Journal of Fujian Forestry Science and Technology

基　　金：华南农业大学博士启动基金(4400-K05023)

摘　　要：从80个随机扩增多态性DNA(RAPD)随机引物中筛选出22个谱带清晰且重复性好的引物用于PCR扩增,探讨夹竹桃科(Apocynaceae)10属12种植物的遗传关系。获得162个位点用于计算Nei’s遗传相似性系数,并应用NTSYS程序UPGMA法构建了系统发育树。黄蝉(Allamanda schottiiPohl.)与软枝黄蝉(A.catharticaL.)、红鸡蛋花(Plumeria rubraL.)与鸡蛋花(P.rubraL.‘Acutifolia’)之间的遗传相似性最高;在遗传相似性系数0.73处将12种植物划分为10个属,并在0.58处分为3支,但并不完全支持3个独立的亚科。RAPD分析结果与形态学的分析结果基本一致,可为夹竹桃科系统分类学提供分子生物学的证据。Twelve species, belonging to 10 genera of Apocynaceae, were analyzed by random amplified polymorphic DNA （RAPD） markers. 162 loci generated by 22 clear and repeated and polymorphic primers, which screened from 80 primers. A close relation was found both between Allarnanda schottii Pohl, and A. cathartica L. and between Plurneria rubra L. and P. rubra L. ＇ A- cutifolia＇. The UPGMA dendrogram, which based on Nei＇ s similarity coefficients and constructed by NTSYS programs, was consistent with that of classical classification. Ten genera were distinguished at the similarity coefficients of 0.73, and 3 branches were distinct at 0.58 while not complete vest in 3 substantive subfamily. Consequently, RAPD is a useful technique in the phylogenetic analysis of Apocynaceae.

关 键 词：夹竹桃科 随机扩增多态性DNA(RAPD) 系统发育 

分 类 号：Q941[生物学—植物学]