冬虫夏草菌丝体水提物抗氧化和保护氧化损伤活性研究  

THE ANTIOXIDANTIVE ACTIVITIES AND OXIDATIVE INJURY PROTECTIVE EFFECTS OF HOT-WATER EXTRACTS FROM CORDYCEPS SINENSIS MYCELIA

作　　者：罗建光[1] 杨晓彤[1] 杨庆尧[1] 糜可[1] 冯慧琴[1] 

机构地区：[1]上海师范大学生命与环境科学学院微生物与免疫学研究所,上海200234

出　　处：《现代预防医学》2007年第18期3433-3435,共3页Modern Preventive Medicine

基　　金：上海市教育委员会科学项目(04DB20);香港保健协会部分资助

摘　　要：[目的]研究冬虫夏草(Cordyceps sinesis)菌丝体热水提物(CS-HWE)抗氧化活性及体外氧化损伤保护作用。[方法]采用铁离子还原法(FRAP)和H2O2氧化损伤的PC12细胞模型对CS-HWE抗氧化活性及对氧化损伤保护活性分别进行了测定。[结果]样品抗氧化活性为(158.03±5.39)μmolFe2+·g-1,相当于天然虫草提取物活性的75%。CS-HWE在50￣100mg·L-1时可明显提高PC12细胞对H2O2氧化损伤的保护作用,活性与天然虫草提取物相当。[结论]虫草菌丝体热水提物具有一定的抗氧化作用,可作为开发天然抗氧化药物的潜在资源。　[Objective]To investigate the activities of CS-HWE,the hot-water extracts derived from the liquid-cultured Cordyceps sinensis mycelia,on antioxidantion and oxidative injury protection.[Methods]The antioxidant activity was detected by using a Ferric Reducing Antioxidant Power assay（FRAP）. The oxidative injury protective effect was assessed by using a PC12 oxidative injury model treated with H2O2,and the survived cells were measured by using MTT assay.[Results]The FRAP assay showed the CS-HWE exerted an antioxidative activity,which was（158.03 ± 5.39）μmol Fe^2＋·g^-1. Its activity was about 75% of that of the whole nature Cordyceps extract was. CS-HWE at concentrations of 50-100 mg·L^-1 could significantly preserve viable PC12 cells after H2O2 treatment,and the protective effect was equivalent to the whole nature Cordyceps extract（P﹤0.05）.[Conclusion]The hot-water extract of Cordyceps sinensis fermentative mycelia possess notable antioxidative activities and has the potential to be used as an antioxidative agent.

关 键 词：冬虫夏草菌丝体 FRAP PC12细胞 抗氧化 

分 类 号：R282.74[医药卫生—中药学]