地肤不同部位提取物对山楂叶螨活性的研究  

Acarocidal Activity of Different Parts of Kochia scoparia against Tetranychus viennensis

作　　者：曹挥[1] 王有年[1] 张铁强[1] 李奕松[1] 刘素琪[2] 李向花[2] 师光禄[1] 

机构地区：[1]农业应用新技术北京市重点实验室,北京102206 [2]山西农业大学农学院,太谷030801

出　　处：《林业科学》2007年第9期27-30,共4页Scientia Silvae Sinicae

基　　金：北京市自然科学基金重点项目(6071001);北京市科委区县专项资金项目(2006);北京市都市农业学科群项目(XK100190553);北京市属市管高校人才强教计划(PXM2007-014207-044536);北京市属市管高校引进人才计划(PXM2007-014207-044538);山西省青年科学基金资助项目(20051036;2006021033)

摘　　要：研究地肤不同部位提取物对山楂叶螨的生物活性。结果表明:地肤子氯仿提取物的杀螨活性最高,浓度为2mg·mL-1对山楂叶螨的卵和雌成螨24h后的校正死亡率分别达到63.37%和70.02%,明显高于根、茎和叶提取物。进一步萃取、柱层析分离发现:萃取物中氯仿相的活性明显大于水相、石油醚相和甲醇相,对氯仿萃取物的柱层析分离最终得到流分11个,其中以第2流分杀螨效果最好,浓度为1mg·mL-1时,24h对卵和雌成螨的校正死亡率分别达68.01%和87.10%。The different parts of Kochia scoparia were investigated for their acarocidal activity against Tetranychus viennensis. The result indicated that the bioactivity of chloroform extract of seed of K. scoparia was the higher than that of root, stem and foliage of K. scoparia, the adjusted mortalities of egg and female mite were 63.37% and 70.02%, respectively at the concentration of 2 mg· mL^-1 . The chloroform extract of seed was then separated and purified. The silica gel column was used to separate and purify active constitutes of the crude extracts of chloroform. A total of 11 bands were seen by TLC analysis. The band 2 appeared a strong acarocidal activity against T. viennensis, the adjusted mortalities of egg and female mite were 68.01% and 87.10% ,respectively at the concentration of 1 mg· mL^-1.

关 键 词：地肤 山楂叶螨 生物活性 

分 类 号：S436.619[农业科学—农业昆虫与害虫防治]