缺氧缺血致低龄大鼠脑白质损伤和神经功能障碍的实验研究  

Cerebral white matter injury induced by hypoxic-ischemia and neurological dysfunction in neonatal rats

作　　者：朱丽华[1] 蒋犁[1] 张志华[1] 朱欢[1] 

机构地区：[1]东南大学临床医学院,江苏南京210009

出　　处：《临床儿科杂志》2007年第9期784-787,共4页Journal of Clinical Pediatrics

摘　　要：目的建立未成熟鼠脑白质损伤模型并研究脑白质损伤与少突胶质细胞间关系。方法3日龄SD大鼠随机分为实验组和假手术组。实验组结扎左侧颈总动脉,术后吸6%氮氧混合气4h,假手术组仅分离左侧颈总动脉,分别于术后24h、48h、72h、11d取脑。采用HE染色、免疫荧光双标记观察新生大鼠脑组织病理变化、少突胶质细胞凋亡;>1月龄鼠测试行为、记忆能力的改变。结果缺氧缺血导致脑白质疏松和脑室扩大等病理变化,深部白质少突胶质细胞凋亡数在损伤后24、48、72h较对照组增多(P<0.05),48、72h两组差异有统计学意义,术后11d两组差异无统计学意义(P>0.05)。模型鼠出现生长发育延迟及神经行为功能缺陷。结论采用3日龄大鼠单侧颈总动脉结扎联合低氧模型研究早产儿脑白质损伤是可行的。Objectives To establish the model of white matter injury in premature rats and to explore the relationship of white matter injury with chattges of the oligodendrocytes, neurobehavioral performance and memory capability. Methods A total of 112 three-day-old Sprague Dawley （SD） premature rats were randomly divided into the experimental group （62） and the control group （50） . The left common carotid arteries were ligated in the rats of the experimental group, and then rats were exposed to 6% oxygen in the containers for 4 hours after 2 hours of recovery period. All rats in two group were killed at 24 h, 48 h, 72 h and 11 days after operation, respectively. The slices of the brains were under- taken with HE staining and double immunofluorescence staining for the observation the pathologic changes of the brain and apoptosis of the oligodendrocytes. Neurobehavioral activity and memory tests were performed at postnatal day 30 （P30） and postnatal day 90 （P90）, respectively. The left common carotid arteries were just separated in the rats of the control group. Results White matter rarefaction and ventricular dilatation induced by hypoxic-ischemia were observed in the experimental group, and there was a significant increase of apoptotic oligodendrocytes in deep white matter at 24 h, 48 h （7.25 ± 0.80 vs 2.30 ±0.39, P 〈 0.05）, and 72 h （6.46 ± 0.68 vs 1.94 ± 0.45, P 〈 0.05） comparing to those in the control group. However, no significant difference in the apoptotic oligodendrocytes was found between two groups on 1 hh day af- ter operation. Furthermore, developmental delay and neurobehavioral dysfunction were predominant in the rats of experimental group. Conclusions It is feasible to use the model of three-day-old SD premature rats with brain damage induced by the ligation of the common carotid artery following four-hour hypoxia for the study of the white matter injury in premature infants.

关 键 词：缺氧缺血 脑室周围白质软化 早产儿 少突胶质细胞 新生大鼠 

分 类 号：R722.6[医药卫生—儿科]