脂膜微泡结合凝血酶原复合物的制备  

The Preparation of Prothrombin Conjugated Lipid Microbubbles

作　　者：杨莉[1] 刘政[2] 左松 谭开彬[2] 高云华[2] 付赤学[2] 李秋颖[2] 

机构地区：[1]解放军第161医院特诊科,武汉430010 [2]第三军医大学新桥医院超声科,重庆400037 [3]爱德华医院功能科,重庆400010

出　　处：《中国医学影像学杂志》2007年第5期321-324,共4页Chinese Journal of Medical Imaging

基　　金：国家自然科学基金项目(No.30471654)

摘　　要：目的:制备结合凝血酶原复合物(PCC)的阴离子脂膜微泡,评价微泡的理化性质。材料和方法:采用机械振荡直接连接法,分两组(于机械振荡之前或之后加入),制备结合PCC的阴离子脂膜微泡,观察并检测洗涤前后微泡的理化性质。结果:微泡与PCC的结合率及Ⅸ因子活性:两组间比较无明显差异(P>0.05),但洗涤后结合率由洗涤前的95%降为80%,活性由90%降为19%。结论:直接连接法可制备结合PCC的阴离子脂膜微泡,微泡与PCC结合率较高,且洗涤前能保持Ⅸ因子较高的活性。Purpose： The purpose of this study is to prepare prothrombin complex concentrate（PCC） conjugated anionic lipid microbubbles and to assess the physiochemical properties of the microbubbles.Materials and Methods： The PCC was directly odded to microbubbles before or after a mechanical vibration.Then the physiochemical properties of the microbubbles were measured and analyzed before or after washing.Results： No significant difference could be found in the binding efficacy and the bio-activity of factor Ⅸ in PCC with or without mechanical vibration.The washing did reduce the binding of fluorescence to MB from 95% to 80%.The activity of factor Ⅸ in PCC dropped from 90% to 19% after three cycles of washing.Conclusion： Prothrombin complex concentrate can be conjugated with lipid microbubbles through direct binding with high efficacy and maintained activities of factor Ⅸ.

关 键 词：脂膜微泡 凝血酶原复合物 直接连接法 理化性质 

分 类 号：R981.9[医药卫生—药品]