化学计量学在秦艽指纹图谱最佳检测波长选择中的应用  

Application of chemometrics in the choice of the best detection wavelength of the fingerprints of gentiana straminea maxim

作　　者：张明锦[1] 

机构地区：[1]青海师范大学化学系,青海西宁810008

出　　处：《计算机与应用化学》2007年第9期1241-1244,共4页Computers and Applied Chemistry

摘　　要：目的:确定中药秦艽HPLC指纹图谱最佳的检测波长。方法:借助于化学计量学信息的提取与处理,建立色谱峰数目、相对峰面积之和以及平均分离度的经验参数CE,以衡量色谱分离的质量,并与色谱峰面积、保留时间与理论塔板数的信息进行比较。结果:两个指标均能如实反映不同检测条件下,秦艽化学特征指纹图谱的分离质量。结论:新建参数CE可客观评价同一检测条件、不同检测波长下,得到中药化学特征指纹图谱的分离质量。Objective：To define the best detection wavelength of the HPLC-FPS of gentiana straminea maxim. Method：By extract and process method of information about to chemometrics, obtain an experience parameter CE that is based on NP （ Number of peak）, SRPA （ Sum of relative area） , SR （Sum of resolution） and used to judge the isolation quality. At same tine, to compared with the index parameter of the function of mutual information that is based on Peak area, Retention time and NTP （number of theoretical plate）. Result ： Two parameters can be better to reflect the isolation quality of the chemical characteristic fingerprint of gentiana straminea maxim. Conclusion： new parameters CE can be used to judge the isolation quality of the chemical characteristic fingerprint of traditional Chinese medicine.

关 键 词：化学计量学 秦艽 指纹图谱 最佳检测波长 

分 类 号：R284.2[医药卫生—中药学]