驯鹿β-防御素-1(reBD-1)基因在组织中表达的原位杂交检测  

Detection of reindeer β-defensin-1(reBD-1) gene's expression in tissues by in situ hybridization

作　　者：杨银凤[1] 赵艳芳 都格尔斯仁[1] 吴萨日娜[1] 

机构地区：[1]内蒙古农业大学动物胚胎与发育生物学研究室,内蒙古呼和浩特010018 [2]呼伦贝尔市鄂温克旗农牧业局,内蒙古鄂温克旗021000

出　　处：《中国预防兽医学报》2007年第9期723-727,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：内蒙古农业大学博士启动基金(BJ04-2);内蒙古自治区自然科学基金资助项目(200508010520)

摘　　要：β-防御素主要是由哺乳动物黏膜上皮细胞产生,分布在机体宿主-环境的界面位置。在本研究中,利用已克隆的驯鹿β-防御素reBD-1cDNA作为探针,经地高辛标记后再应用原位杂交技术检测reBD-1mRNA在驯鹿组织内的表达部位。结果显示reBD-1mRNA表达在舌背表面的复层扁平上皮内,实质性器官肾也表达在肾小管及肾小囊上皮内,而淋巴器官脾脏内reBD-1mRNA表达在淋巴细胞和红髓的巨噬细胞内。表明驯鹿β-防御素不仅表达在上皮细胞内,而且在非上皮细胞内也有表达,提示reBD-1在黏膜和系统防御中均起着重要的作用。Mammalian β-defensins are produced primarily by epithelial cells lining the respiratory, digestive, and urogenital tracts and distribute the interface of organism and environment. In this study we examined the expressive tissues of reBD-1 mRNA in reindeers by RNA In Situ Hybridization using the digoxigenin-labeled reBD-1 nucleic acid probe. The reBD-1 mRNA was detected in the stratified squamous epithelium of dorsal tongue, the renal capsule and tubule epithelium of kidney and in the lymphocytes and microphages of spleen, suggesting that the reindeer β-defensin was expressed not only in epithelium but also in non-epithelial cells. The results indicate that reBD-1 may play an important role both in mucous and systemic defense.

关 键 词：Β-防御素 驯鹿 原位杂交 

分 类 号：Q786[生物学—分子生物学]