HPLC-ECD检测羟自由基方法的研究  

Study on detection of hydroxyl free radical by HPLC-ECD

作　　者：杨威[1] 邓玉林[1] 

机构地区：[1]北京理工大学生命科学与技术学院,北京100081

出　　处：《分析试验室》2007年第9期120-122,共3页Chinese Journal of Analysis Laboratory

摘　　要：采用高效液相色谱结合库仑阵列多电极检测器体系（HPLC—ECD）建立了一套操作简便、高效、快速测定羟自由基的方法，并探究了检测的灵敏性，浓度和响应因子之间的线性关系，系统的稳定性等。2，3-二羟基苯甲酸（2，3-DHBA）、2，5-二羟基苯甲酸（2，5-DHBA）以及水杨酸（sA）的相关系数分别为0．9999、0．9996、0．9968。2，3-DHBA和2，5-DHBA的检出限都低于0．1ng／mL，SA的检出限均低于10ng／mL。各种物质的方法回收率为100．5％。102．9％。High performance liquid chromatography （HPLC-ECD） was used for measurement of the neurotrasmitters. In our study, the method of measuring hydroxyl free radicals was established, and various factors involved in contributing to the reliability and reproducibility of the neurotrasmitters measurement were systematically investigated. These factors include sensitivity of detection, linear relationship between concentration and the response factor, stability of the system. Correlation coefficients of 2,3-DHBA, 2,5-DHBA and SA are 0.9999, 0.9996 and 0.9968, respectively. The limit of detection of 2,3-DHBA and 2,5-DHBA are below 0.1 ng/mL, and SA is below 10 ng/mL.

关 键 词：羟自由基 HPLC—ECD 

分 类 号：O657.7[理学—分析化学]