Effects of UVB on the expression of proliferating cell nuclear antigen (PCNA) in dinoflagellate Prorocentrum donghaiense Lu  

作　　者：LIU Jingwen JIAO Nianzhi CAI Huinong HONG Huasheng 

机构地区：[1]Bioengineering School of Jimei University, Xiamen 361021, China [2]State Key Laboratory of Marine Environmental Sciences of Xiamen University, Xiamen 361005, China

出　　处：《Acta Oceanologica Sinica》2007年第4期123-131,共9页海洋学报（英文版）

基　　金：the National Key Basic Research and Development Program （red tide ＂973＂program） of China under contract No. 2301CB409700; the National Natural Science Foundation of China under contract Nos 40232021,405211303; Ministry of Science and Technology of China under contract Nos 2005AA635240 and MEL0608.

摘　　要：PCNA has been considered as a useful marker for the estimation of growth rates of marine phytoplankton at the species level. Since dinoflagellates are noted for having many prokaryotic features in that they are the only eukaryotes to have permanently condensed chromosomes as well as lacking histones and a nucleosome, the sensitivity to UVB radiation and the validity of PCNA as a maker of growth rate in dinoflagellate need to be evaluated. Prorocentrum donghaiense Lu was investigated to valuate the UVB sensitivity in relation to cellular and molecular aspects of PCNA as a growth indicator. The effects of UVB radiation on PCNA were studied using the methods of western blots technology and whole-cell immunoflurescence with one mono-antibody. UVB changed the cell morphology, halted the growth and increased the cell size, even caused cell death to a certain extent after treatment with UVB radiation in P. donghaiense. Compared with the control, treating the algal cultures in exponential phases with UVB radiation for 24 h caused chromatin release and increases in protein levels of PCNA.PCNA has been considered as a useful marker for the estimation of growth rates of marine phytoplankton at the species level. Since dinoflagellates are noted for having many prokaryotic features in that they are the only eukaryotes to have permanently condensed chromosomes as well as lacking histones and a nucleosome, the sensitivity to UVB radiation and the validity of PCNA as a maker of growth rate in dinoflagellate need to be evaluated. Prorocentrum donghaiense Lu was investigated to valuate the UVB sensitivity in relation to cellular and molecular aspects of PCNA as a growth indicator. The effects of UVB radiation on PCNA were studied using the methods of western blots technology and whole-cell immunoflurescence with one mono-antibody. UVB changed the cell morphology, halted the growth and increased the cell size, even caused cell death to a certain extent after treatment with UVB radiation in P. donghaiense. Compared with the control, treating the algal cultures in exponential phases with UVB radiation for 24 h caused chromatin release and increases in protein levels of PCNA.

关 键 词：UVB radiation proliferating cell nuclear antigen （PCNA） dinoflagellate Prorocentrum donghaiense Lu 

分 类 号：Q178.53[生物学—水生生物学]