锰(Ⅲ)Corrole配合物催化DNA氧化断裂  

Oxidative DNA Cleavage Catalyzed by Mn(Ⅲ) Corroles

作　　者：刘海洋[1] 刘兰英[1] 张雷[2] 应晓[3] 王湘利[1] 江焕峰[1] 张启光[4] 

机构地区：[1]华南理工大学化学系,广州5106412 [2]华南理工大学制药工程系,广州510641 [3]华南理工大学应用物理系,广州510641 [4]香港科技大学化学系

出　　处：《高等学校化学学报》2007年第9期1628-1630,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20332030;20572027;N-HKUST604/04);教育部留学回国人员科研启动基金(批准号:2004527)资助.

摘　　要：Several Mn(Ⅲ)10-(hydroxylphenyl)-5,15-bis(pentafluoro-phenyl)corroles with the hydroxyl at ortho-,meta-and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H2O2.The catalytic system was composed of 10 μL 0.5 mg/mL pC DNA 6 in TE buffer solution(pH=8.0,4.0×10-2 mol/L Tris,1.0×10-3 mol/L EDTA),10 μL 1.0×10-5 mol/L Mn(Ⅲ)corrole in DMSO and 10 μL 0.3% H2O2.After 8 h incubation at room temperature,significant oxidative DNA damage could be observed with the ortho-hydroxyl Mn(Ⅲ)corrole showing a better activity as detected by agarose-gel electrophoresis.Plasmid DNA was completely damaged after 12 h incubation.These observations show Mn(Ⅲ)corrole can be used as an artificial model of nuclease.Several Mn（ Ⅲ） 10-（hydroxylphenyl）-5,15-bis（pentafluoro-phenyl） corroles with the hydroxyl at ortho-, meta- and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H202. The catalytic system was composed of 10 μL 0. 5 mg/mL pC DNA 6 in TE buffer solution（pH =8.0, 4.0 ×10^-2 mol/L Tris, 1.0 ×10^-3 mol/L EDTA）, 10 μL 1.0 ×10^-5 mol/L Mn（ Ⅲ） corrole in DMSO and 10 μL 0.3% H2O2. After 8 h incubation at room temperature, significant oxi- dative DNA damage could be observed with the ortho-hydroxyl Mn （Ⅲ ） corrole showing a better activity as detected by agarose-gel electrophoresis. Plasmid DNA was completely damaged after 12 h incubation. These observations show Mn（Ⅲ） corrole can be used as an artificial model of nuclease.

关 键 词：锰 CORROLE DNA 氧化断裂 

分 类 号：O611.3[理学—无机化学]