高效液相色谱法测定小鼠肿瘤中β-榄香烯的含量  

Determination of β-elemene in mouse tumor by HPLC

作　　者：王艳芝[1] 郑甲信[1] 邓意辉[2] 史启君 毕殿洲[2] 

机构地区：[1]郑州大学药学院,河南郑州450001 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]北京紫竹药业有限公司,北京100081

出　　处：《中国医院药学杂志》2007年第9期1246-1248,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的：建立小鼠肿瘤中β-榄香烯的含量分析方法并测定静脉给予β-榄香烯固体脂质纳米粒和乳剂1h后小鼠肿瘤中β-榄香烯的浓度。方法：采用HPLC法，色谱柱为Hypersil BDS C18（4．6mm×200mm，5μm）；流动相；乙腈-水（90：10）；检测波长为208nm；流速为1mL·min^-1。建立小鼠皮下移植性H22肿瘤模型。结果：β-榄香烯与肿瘤中其他组分能很好分离，在0.4～24．0mg·L^-1“范围内线性关系良好（r=0．9995）。相对回收率大于97％。结论：本方法简单、准确、专属性强，可用于测定实体肿瘤中β-榄香烯的含量。OBJECTIVE An HPLC method was developed for determination of β-elemene in tumor. Concentrations of β-elemene at 1 h after iv administration of solid lipid nanoparticles （SLN） and emulsion to H22-bearing mice were determined respectively. METHODS The detection was carried out on Hypersil BIDS C18 （ 4. 6 mm × 200 mm,5μm） by using acetonitrile-water（90： 10） as the mobile phase with detection at 208 nm and the flow rate was 1.0 mL·min^-1. Tumor-bearing mice were established by injecting H22 cells subcutaneously at the right axillary flank of male KM mice. RESULTS β-elemene could be well separated from other components. The linear range was 0. 4-24. 0 mg·L^-1 （r = 0. 999 5）, the average recovery was higher than 97%. CONCLUSION This method is accurate and simple in detecting tumor concentration of β-elemene.

关 键 词：Β-榄香烯 固体脂质纳米粒 乳剂 高效液相色谱 H22肿瘤 

分 类 号：R927.2[医药卫生—药学]