氯化两面针碱体外诱导人口腔鳞癌KB细胞G_2/M期阻滞及凋亡的研究  

Study on G_2/M Phase Arrest and Apoptosis of Human Carcinoma of Mouth Floor KB Cells in vitro Induced by Nitidine Chloride

作　　者：刘华钢[1] 王博龙[1] 秦三海[1] 杨斌[1] 

机构地区：[1]广西医科大学,广西南宁530021

出　　处：《时珍国医国药》2007年第9期2104-2106,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广西科学研究与技术开发计划项目(桂科攻No.063002-2E);广西大型仪器协助共用网测试及仪器运行补助经费资助项目

摘　　要：目的探讨氯化两面针碱对人口腔鳞癌KB细胞周期和凋亡的影响及相互关系。方法应用MTT比色法测定氯化两面针碱对细胞增殖的抑制作用;流式细胞术(FCM)检测氯化两面针碱对细胞周期的影响;Hoechst33258检测细胞凋亡;透射电镜观察细胞超微结构。结果氯化两面针碱在体外呈浓度依赖性显著抑制KB细胞的生长,48hIC_(50)为(2.36±0.22)μg/ml;与空白组相比,经3μg/ml氯化两面针碱作用,G_2/M期细胞比例显著增多;6μg/ml时凋亡细胞比例最高,可见典型的核染色质凝集、碎裂;9μg/ml时,细胞凋亡率明显下降,同时镜下可见大量坏死细胞。结论氯化两面针碱抗KB细胞的方式与其剂量密切相关,低浓度时以G_2/M期阻滞为主,中浓度时诱导凋亡,高浓度时则致其坏死。Objective To explore the influence of nitidine chloride on cell cycle and apoptosis of human carcinoma of mouth floor KB cells and their relationship. Methods The proliferation inhibition rate and IC50 （48 h） of nitidine chloride to KB cells were examined by MTF assay. The influence of nitidine chloride on cell cycle was measured by flow eytometry, Hoechst33258 technology was adopted to detect cell apoptosis. Transmission electron microscope was used to observe cell ultrastructure, Results Nitldine chloride markedly suppressed proliferation of KB cells in a dose - dependent manner in vitro, IC50 for 48h was （2.36 ±0.22） μg/ ml. Compared with control group, after treated with 3bLg/ml of nitidine chloride,the percentage of G/M phase cells significantly increased. The highest apoptotic percentage and typical nuclear chromatine condensation and fragmentation were observed at the nitidine chloride concentration of 6 μg/ml, When KB cells were treated with nitidine chloride of 9 μg/ml,cell apoptosls rate significantly decreased , many necrotic cells were seen under transmission electron microscope at the same time. Conclusion The means of nitidine chloride inhibiting KB cells is closely associated with its dose,nitidine chloride induced G2/M phase arrest at low concentration, induced apoptosis at medium concentration and lead to necrosis at high concentration.

关 键 词：氯化两面针碱 G2/M期阻滞 凋亡 坏死 KB细胞 

分 类 号：G962[文化科学]