红景天注射液的提取与精制工艺研究  

Study on the Extraction and Purification Process of Hongjingtian Injection

作　　者：杨婷媛[1] 李三鸣[1] 刘洪卓[1] 姚慧敏[1] 李红菊[1] 

机构地区：[1]沈阳药科大学,辽宁沈阳110016

出　　处：《时珍国医国药》2007年第9期2227-2228,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的对红景天注射液的提取与精制工艺进行研究。方法以红景天苷和酪醇的总转移率为考察指标,采用正交实验对提取与精制工艺进行优化。结果红景天的最佳提取工艺为:将粉碎后的药材,以8倍量的水为提取溶剂,加热回流提取3次,1h/次;采用醇沉法和明胶沉淀法对红景天水提取物进行精制,醇沉法中调含醇量至70%,明胶沉淀法的最佳工艺为:将醇沉后的药液浓缩至0.5mg·ml^(-1)(相当于原生药),调pH值至5.0,在60℃,转速为60r·min^(-1)的条件下,加入5%明胶溶液至不再生成沉淀时为止。结论采用上述提取精制方法制得的红景天浸膏杂质检查符合注射液的要求,该方法可用于红景天注射液的提取与精制。Objective To study the extraction and purification process of Hongjingtian Injection. Methods An orthogonal method was adopted to optimize the extraction and purification process with the transference rate of Salidroside and p - Tyrosol as criterion. Results The optimized process of extraction was that eight times water as extracted solvent, the comminuted medical material was extracted three times,each for an hour. Alcohol precipitation method and gel flocculating method were both used to optimize purification process. Alcohol precipitated concentration was 70%. The optimized process of gel flocculating method was that the extracted solution was condensed to 0, 5g ·ml^-1 （ equivalent to raw material ） and its pH was adjusted to 5, 0, The 5, 0% gelatin solution was added into the condensed solution with agitation rate of 60 r ·min^ - l at 60℃ until there was no more precipitation. Conclusion The results of impurity test meet the standard for injection. The established methods can be used for extraction and purification of Hongjingtian injection.

关 键 词：红景天注射液 提取 精制 红景天苷 酪醇 

分 类 号：R284.2[医药卫生—中药学]