IL-13对LPS诱导人系膜细胞分泌IL-12的影响  

Effects of interleukin-13 on interleukin-12 production in mesangial cells induced by LPS

作　　者：吴瑗[1] 刘华锋[2] 江黎明[2] 陈孝文[2] 何惠娟[2] 

机构地区：[1]广东医学院微生物与免疫学教研室,广东湛江524001 [2]广东医学院附属医院肾脏病研究所,广东湛江524001

出　　处：《中国病理生理杂志》2007年第9期1801-1803,共3页Chinese Journal of Pathophysiology

摘　　要：目的:探讨白细胞介素13(IL-13)对肾小球系膜细胞分泌白细胞介素12(IL-12)的影响作用。方法:用酶联免疫吸附(ELISA)法和逆转录聚合酶链反应(RT-PCR)法检测系膜细胞IL-12蛋白和L-12p40mRNA表达。结果:LPS诱导系膜细胞的IL-12p40mRNA表达及其蛋白分泌(P<0.01)。IL-13在1-100μg/L浓度范围内呈剂量依赖性抑制LPS诱导的系膜细胞IL-12分泌及其mRNA表达(P<0.05或P<0.01)。结论:IL-13可能通过抑制LPS诱导系膜细胞分泌IL-12,而调整了体内Th1/Th2细胞因子平衡。AIM： To investigate the effect of interleukin - 13 （ IL - 13 ） on interleukin - 12 （ IL - 12 ） production in mesangial cells. METHODS： The protein synthesis of IL - 12 in mesangial cells was measured by ELISA. The expression of IL - 12 mRNA in mesangial cells was evaluated by RT - PCR. RESULTS： The production of IL - 12 in mesangial ceils stimulated by lipepelysaccharide（LPS） was significantly increased （ P 〈 0.01 ）. IL - 13 （ 1 - 100 μg/L） inhibited the protein and mRNA expression of IL - 12 in a dose - dependent manner （ P 〈 0. 05 or P 〈 0. 01 ）. CONCLUSION： IL - 13 inhibits IL - 12 expression induced by LPS in mesangial cells. IL - 13 may regulate immune responses by balancing Th1/Th2 in glomerulonephritis.

关 键 词：白细胞介素13 白细胞介素12 系膜细胞 脂多糖类 

分 类 号：R692[医药卫生—泌尿科学]