苏云金芽孢杆菌aiiA基因的克隆与结构分析  

Cloning and Analysis of aiiA Gene from Bacillus thuringiensis

作　　者：杨梅[1] 林彬辉[1] 关夏玉[1] 郭丽清[1] 姚帆[1] 黄天培[1] 黄志鹏[1] 关雄[1] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室

出　　处：《福建师范大学学报（自然科学版）》2007年第5期85-90,共6页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省科技厅基金资助项目(2005K019Z05160072006J0051)

摘　　要：分别克隆了叶片、苔藓和土壤中分离的Bacillus thuringiensis的aiiA基因,并对其编码的蛋白AiiA-P28、AiiA-B19和AiiA-S2进行了理化特征分析、进化树分析和空间结构的研究.结果表明AiiA-P28、AiiA-B19和AiiA-S2分子质量均为28 ku;等电点分别为4.77、4.77、4.93;三者均为亲水性蛋白,具有很高的同源性.进化树分析结果表明叶片中分离的AiiA-P28与苔藓中分离的AiiA-B19进化距离较近,而土壤中分离的AiiA-S2则与它们的进化距离较远.三维结构分析表明,AiiA蛋白具有典型的αβ/βα折叠的空间结构.The aiiA genes of Bacillus thuringiensis which were isdated from leaves, bryopsida and soil were cloned. The results showed that the deduced molecular weight of three AliA proteins were about 28 ku with the isoelectric point of 4, 77, 4.77 and 4.93 respectively. All of them were hydrophilic proteins. Phyloge- netic analysis showed AiiA proteins were highly conserved. The AiiA-P28 shared closer genetic relationship with AiiA-B19 compared with AiiA-S2. Three-dimensional structure analysis showed all AiiA proteins share an αβ/βα fold.

关 键 词：苏云金芽孢杆菌 AIIA基因 进化树分析 空间结构 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治]