大肠杆菌E．coli K-12转录因子结合位点的理论预测  

Recognition of the transcription factor binding sites in Escherichia coli K-12

作　　者：杨科利[1] 李前忠[1] 林昊[1] 

机构地区：[1]内蒙古大学理工学院物理系,内蒙古呼和浩特010021

出　　处：《生物信息学》2007年第3期117-120,共4页Chinese Journal of Bioinformatics

基　　金：国家自然科学基金项目(No.30560039)

摘　　要：基于实验验证的22种大肠杆菌K12的转录因子结合位点序列,分析了转录因子结合位点每一位置的碱基保守性,提出了预测转录因子结合位点的位置权重矩阵打分函数算法(PWMSA)。利用self-consistency和cross-validation两种检验方法对此算法进行检验,self-consistency检验总的预测成功率达到87.59%,cross-validation检验成功率达到85.48%。对基因间序列进行搜索,获得了多个可能的转录因子结合位点。Bascd on the known transcription factor binding sites in Eacherichia coli K- 12 genome, the conservation analysis of transcription factor binding sites shows that the conservative nuclotide in different sites play a key role in transcription factor binding sites regions, and a novel position - weight scoring matrix scoring algorithm for predicting transcription factor binding sites is presented. By using of the self- consistency test and the 10- fold cross- validation test, the results show that the overall prediction accuracies are 87.59% and 85.48%, respectively. In addition, some possible transcription factor binding sites are predicted by searching for the intergenic regions.

关 键 词：转录因子结合位点(IFBS) 位置权重矩阵(PWM) 碱基保守性 

分 类 号：Q61[生物学—生物物理学]