肺孢子虫肺炎大鼠支气管肺泡灌洗液酶含量变化及大蒜素治疗对其影响  

Enzyme Change in Bronchoalveolar Lavage Fluid of Pneumocystis Pneumonia Rats and the Effect of Garlicin Treatment

作　　者：卢致民[1] 张有成[2] 张进顺[1] 史宝林[3] 贾天军[3] 唐红炜[1] 刘金花[1] 

机构地区：[1]河北北方学院寄生虫学教研室,张家口075000 [2]河北北方学院附属第一医院检验科,张家口075000 [3]河北北方学院检验系,张家口075000

出　　处：《中国寄生虫学与寄生虫病杂志》2007年第4期300-303,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：河北省卫生厅资助项目(No.05094)~~

摘　　要：目的研究卡氏肺孢子虫肺炎(PCP)大鼠支气管肺泡灌洗液(BALF)酶含量变化以及大蒜素治疗对其影响。方法用地塞米松连续肌肉注射Wistar大鼠,诱导建立PCP大鼠模型。诱导第3、6、9周分别于后腿肌肉深部注射大蒜素治疗(10mg/kg,1次/d,连续5d)。同时设甲氧苄氨嘧啶(TMP)-磺胺甲基异噁唑(SMZ)治疗对照组(SMZ/TMP组)、PCP模型对照组和空白对照组。于最后1次治疗3d后处死大鼠,无菌收集BALF,测定谷草转氨酶(AST)、谷丙转氨酶(ALT)、胆碱脂酶(CHE)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、肌酸激酶(CK)及其同工酶(CKMB)、a-羟丁酸脱氢酶(HBDH)、a-L-岩藻糖甘酶(AFU)、5′-核苷酸酶(5′NT)及腺苷脱氨酶(ADA)含量。结果PCP模型组ALP含量[(573.41±350.63)U/L]显著高于空白对照组[(210.56±114.41)U/L](q=4.682,P<0.01)、大蒜素治疗组[(392.07±217.57)U/L](q=3.851,P<0.05)以及SMZ/TMP组[(325.21±180.65)U/L](q=4.380,P<0.01)。CK、CKMB及5′NT含量,PCP模型对照组[依次为948.94±403.43、489.47±254.46及(6.76±3.11)U/L]显著高于空白对照组[426.22±319.00、213.33±144.54及(3.22±1.20)U/L](q=4.696,3.784,3.812,P<0.05)。AST、ALT、CHE、LDH、HBDH、AFU及ADA含量,4组之间差异均无统计学意义(F=1.852,0.958,2.470,1.423,1.178,1.342,0.611,P>0.05)。结论PCP大鼠BALF中ALP、CK、CKMB及5′NT含量显著升高,大蒜素治疗可使ALP含量显著降低。　Objective To study the change of enzymes and effect of garlicin treatment on the change in bronchoalveolar lavage fluid（BALF） of rats with Pneumocystis carinii pneumonia （PCP）. Methods Wistar rats were injected intramuscularly continually with dexamethasone to establish the rat model of PCP. The experimental rats （group A） were injected intramuscularly with garlicin at a dose of 10 mg/（kg·d） for 5 days in the 3rd,6 th and 9th week respectively,and SMZ/TMP therapy group （B）,PCP infected group （C） and normal group （D） were established as controls. Three days after the last treatment,the rats of all groups were killed and BALF was collected without contamination and enzymes AST,ALF,CHE,ALP,LDH,CK,CKMB,HBDH,AFU,5′NT,ADA were examined. Results The ALP level in group C （573.41 ± 350.63）U/L was significantly higher than that in group D （210.56 ± 114.41）U/L （q=4.682,P〈0.01）,group A （392.07 ± 217.57）U/L （q=3.851,P〈0.05）,and group B （325.21 ± 180.65）U/L （q=4.380,P〈0.01）; the level of CK,CKMB and 5′NT in group C 948.94 ± 403.43、 489.47 ± 254.46 and （6.76 ± 3.11）U/L respectively was higher than those in group D 426.22 ± 319.00,213.33 ± 144.54 and （3.22 ± 1.20）U/L （q=4.696,3.784,3.812,P〈0.05）; there was no significant difference in the level of AST,ALT,CHE,LDH,HBDH,AFU and ADA among the four groups（F=1.852,0.958,2.470,1.423,1.178,1.342,0.611,P〉0.05）. Conclusions The level of ALP,CK,CKMB and 5′NT increases evidently in BALF of PCP infected rats,but the ALP level decreases distinctly after the garlicin treatment.

关 键 词：肺孢子虫肺炎 支气管肺泡灌洗液 酶 大鼠 

分 类 号：R531.5[医药卫生—内科学]