尼古丁降解菌的分离筛选及初步鉴定  

Isolation and Identification of Nicotine-degrading Microbes

作　　者：韩绍印[1] 李永宽 席宇[1] 杨艳坤[1] 宋淑红[1] 朱大恒[1] 

机构地区：[1]郑州大学生物工程系,河南郑州450052 [2]郑州卷烟总厂,河南郑州450000

出　　处：《河南农业科学》2007年第9期48-51,共4页Journal of Henan Agricultural Sciences

基　　金：河南省自然科学基金项目(511030400);河南省重点科技攻关项目(072102170010)

摘　　要：分别采用基础平板培养法和富集培养法,从河南烟叶表面分离筛选尼古丁降解菌。结果表明:采用基础平板培养法分离出来的30个菌株中,有效菌株仅占所分离菌株的60.0%,对尼古丁降解效率大于30%的仅有6株菌(37℃,24 h)。而富集培养法分离出来的30个菌株中,有效菌株占所分离菌株的比例达93.3%,对尼古丁降解效率大于30%的有14株菌,表明富集培养法是一种快速高效分离筛选尼古丁降解菌的理想方法。经初步鉴定,分离到的2株具有较高活性的尼古丁降解菌分别为短芽孢杆菌(B.brevis)和侧孢芽孢杆菌(B.laterosporus)。Nicotine-degrading microbes were Isolated from Henan tobacco leaves by basic culture and enrichment culture methods. The results showed that the effect of enrichment culture is better than that of basic culture in isolation and selection of nicotine degrading microbes. The ratio of valid strains isolated by basic culture method was 60.0% ,while that by enrichment culture method was 93.3%. The stain number with nicotine degradation rate of more than 30 % was 6 out of the 30 strains isolated from the basic culture, and 14 from the enrichment culture. Based on th physiology and biochemistry characters, 2 strains with higher nicotine-degrading activity were identified, i. e. B. brevis and B. laterosporus.

关 键 词：微生物 烟草 尼古丁 降解 分离 鉴定 

分 类 号：S154.3[农业科学—土壤学]