南阳牛遗传多样性的RAPD分析  

RAPD Analysis of Genetic Diversity in Nanyang Cattle

作　　者：梁子安[1] 鲁云风[1] 王庆林[1] 

机构地区：[1]南阳师范学院生命科学与技术学院,河南南阳473061

出　　处：《河南农业科学》2007年第9期99-100,103,共3页Journal of Henan Agricultural Sciences

基　　金：河南省自然科学基金项目(0411034200)

摘　　要：用RAPD技术对南阳牛种质资源的DNA进行检测,采用Popgene软件对扩增结果进行统计分析表明:筛选的16个引物共得到了236条扩增DNA片断,其中89.7%的扩增条带表现出多态性。平均每个位点有1.989 6个等位基因,有效等位基因数为1.233 3。Nei遗传多样性指数为0.159 7,Shannon's遗传多样性指数为0.270 3。种群总的遗传多样性Ht为0.163 7,种群内的遗传多样性Hs为0.130 5,种群的基因分化系数为0.202 8,种群间的基因流为1.965 3。The genetic diversity of Nanyang cattle accessions was tested by RAPD technology and the Popgene software. The result showed that a total of 236 bands were amplified by 16 mer arbitrary primers,of which 89.7% bands were polymophic. In average, 1. 9896 alleles was found in each locus,of which the number of effective allele is 1. 233 3. The index of genetic diversity for Nei and Shannon＇s was 0. 159 7 and 0. 270 3, respectively. Ht was 0. 163 7 and Hs 0. 130 5. The gene differentiation coefficient in populations is 0. 202 8 and gene class between populations is 1. 965 3.

关 键 词：南阳牛 RAPD 遗传多样性 

分 类 号：S823.811[农业科学—畜牧学]